Sorted By Test Name - Mayo Medical Laboratories

80027
IABCS
88800
patients with B-cell lymphoma and track the response to therapy.
Interpretation: A neoplastic clone is detected when the percent of cells with an abnormality exceeds
the normal reference range for any given probe. The absence of an abnormal clone does not rule the
presence of neoplastic disorder.
Reference Values:
An interpretive report will be provided.
Clinical References: 1. Remstein ED, Kurtin PJ, Buno I, et al: Diagnostic utility of fluorescence in
situ hybridization in mantle-cell lymphoma. Br J Haematol 2000 Sep;110(4):856-862 2. Remstein ED,
Kurtin PJ, James CD, et al: Mucosa-associated lymphoid tissue lymphomas with t(11;18) (q21;q21) and
mucosa-associated lymphoid tissue lymphomas with aneuploidy develop along different pathogenetic
pathways. Am J Pathol 2002 Jul;161(1):63-71 3. Remstein ED, Dogan A, Einerson RR, et al: The
incidence and anatomic site specificity of chromosomal translocations in primary extranodal marginal
zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) in North America. Am
J Surg Pathol 2006 Dec;30(12):1546-1553H636
B-Cell Lymphoma, FISH, Tissue
Clinical Information: Lymphoid neoplasms are known to be complex and the prognosis and clinical
course of patients with lymphoma is highly variable. Genetic abnormalities have emerged as 1 of the most
reliable criteria for categorizing lymphomas. Several chromosome anomalies and variants of these
anomalies have been associated with various kinds of lymphoma (see table). Unfortunately, conventional
chromosome studies cannot be employed on paraffin-embedded tissue and molecular genetic analyses are
often problematic in the study of lymphomas. New methods using chromosome-specific
fluorescent-labeled DNA probes and FISH now permit detection of abnormal genes associated with
various chromosome anomalies in lymphoma (see table). Common Chromosome Anomalies in
Lymphomas Lymphoma Type Chromosome Anomaly FISH Probe Burkitt t(8;14)(q24;q32) MYC/IGH
t(8;22)(q24;q11.2) MYC/IGL t(22;var)(q11.2;var) IGL Mantle Cell t(11;14)(q13;q32) CCND1/IGH
Follicular t(14;18)(q32;q21) IGH/BCL2 Diffuse large B-cell t(3;var)(q27;var) BCL6 t(14;18)(q32;q21)
IGH/BCL2 t(8;14)(q24;q32) MYC/IGH t(8;22)(q24;q11.2) MYC/IGL t(22;var)(q11.2;var) IGL
Useful For: Detecting chromosome anomalies in paraffin-embedded lymphoid tissue Providing
diagnostic and prognostic genetic information for the following lymphomas: mantle cell, follicular,
Burkitt, and diffuse large B-cell lymphoma We recommend this test in cases where the diagnosis is
questionable, or potential prognostic information may become available
Interpretation: A neoplastic clone is detected when the percent of cells with an abnormality exceeds
the normal reference range for any given probe.
Reference Values:
An interpretive report will be provided.
Clinical References: 1. Jaffe ES: World Health Organization of Tumours. Pathology and Genetics
of Tumours of Haematopoietic and Lymphoid Tissues. International Agency for Research on Cancer 2001
2. Paternoster SF, Brockman SR, McCLure RF, et al: A new method to extract nuclei from
paraffin-embedded tissue to study lymphomas using interphase fluorescence in situ hybridization. Am J
Pathol 2002;160(6):1967-1972 3. Remstein ED, Kurtin PJ, Buno I, et al: Diagnostic utility of fluorescence
in situ hybridization in mantle-cell lymphoma. Br J Haematol 2000;110(4):856-862 4. Remstein ED,
Kurtin PJ, James CD, et al: Mucosa-associated lymphoid tissue lymphomas with t(11;18)(q21;q21) and
mucosa-associated lymphoid tissue lymphomas with aneuploidy develop along different pathogenetic
pathways. Am J Pathol 2002;161:63-71
B-Cell Phenotyping Profile for Immunodeficiency and Immune
Competence Assessment, Blood
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