Sorted By Test Name - Mayo Medical Laboratories

TBBS
9336
2-5 years: 700-2,200 cells/mcL*
6-11 years: 650-1,500 cells/mcL*
12-17 years: 530-1,300 cells/mcL*
18-55 years: 424-1,509 cells/mcL
>55 years: 430-1,513 cells/mcL
Suppressor Cells (CD8)
0-2 months: 560-1,700 cells/mcL*
3-5 months: 590-1,600 cells/mcL*
6-11 months: 500-1,700 cells/mcL*
12-23 months: 620-2,000 cells/mcL*
2-5 years: 490-1,300 cells/mcL*
6-11 years: 370-1,100 cells/mcL*
12-17 years: 330-920 cells/mcL*
18-55 years: 169-955 cells/mcL
>55 years: 101-839 cells/mcL
LYMPHOCYTE RATIO
H/S ratio: > or =1.0
*Shearer WT, Rosenblatt HM, Gelman RS, et al: Lymphocyte subsets in healthy children from birth
through 18 years of age: The Pediatric AIDS Clinical Trials Group P1009 study. J Allergy Clin Immunol
2003;112(5):973-980
LYMPHOCYTE PROLIFERATION TO MITOGENS
Viability of lymphocytes at day 0: > or =75.0%
Maximum proliferation of phytohemagglutinin as % CD45: > or =49.9%
Maximum proliferation of phytohemagglutinin as % CD3: > or =58.5%
Maximum proliferation of pokeweed mitogen as % CD45: > or =4.5%
Maximum proliferation of pokeweed mitogen as % CD3: > or =3.5%
Maximum proliferation of pokeweed mitogen as % CD19: > or =3.9%
Clinical References: See individual test IDs.
T- and B-Cell Quantitation by Flow Cytometry
Clinical Information: Normal immunity requires a balance between the activities of various
lymphocyte subpopulations with different effector and regulatory functions. Different immune cells can
be characterized by unique surface membrane antigens described by a cluster of differentiation
nomenclature (eg, CD3 is an antigen found on the surface of T lymphocytes). Abnormalities in the
number and percent of T (CD3), T-helper (CD4), T-suppressor (CD8), B (CD19), and natural killer
(CD16+CD56) lymphocytes have been described in a number of different diseases. In patients who are
infected with HIV, the CD4 count is measured for AIDS diagnosis and for initiation of antiviral therapy.
The progressive loss of CD4 T lymphocytes in patients infected with HIV is associated with increased
infections and complications. The Public Health Service has recommended that all HIV-positive patients
be tested every 3 to 6 months for the level of CD4 T lymphocytes. The absolute counts of lymphocyte
subsets are known to be influenced by a variety of biological factors, including hormones, the
environment, and temperature. The studies on diurnal (circadian) variation in lymphocyte counts have
demonstrated progressive increase in CD4 T-cell count throughout the day, while CD8 T cells and CD19+
B cells increase between 8:30 am and noon, with no change between noon and afternoon. Natural killer
(NK) cell counts, on the other hand, are constant throughout the day.(1) Circadian variations in circulating
T-cell counts have been shown to be negatively correlated with plasma cortisol concentration.(2-4) In
fact, cortisol and catecholamine concentrations control distribution and, therefore, numbers of naive
versus effector CD4 and CD8 T cells.(2) It is generally accepted that lower CD4 T-cell counts are seen in
the morning compared with the evening,(5) and during summer compared to winter.(6) These data,
therefore, indicate that timing and consistency in timing of blood collection is critical when serially
monitoring patients for lymphocyte subsets.
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