Sorted By Test Name - Mayo Medical Laboratories

CHIDB
83182
CHEP
84427
1 0.35-0.69 Equivocal
2 0.70-3.49 Positive
3 3.50-17.4 Positive
4 17.5-49.9 Strongly positive
5 50.0-99.9 Strongly positive
6 > or =100 Strongly positive Reference values
apply to all ages.
Clinical References: Homburger HA: Allergic diseases. In Clinical Diagnosis and Management by
Laboratory Methods. 21st edition. Edited by RA McPherson, MR Pincus. New York, WB Saunders
Company, 2007, Chapter 53, Part VI, pp 961-971
Chimerism-Donor
Clinical Information: See CHEP/84427 Chimerism-Recipient Engraftment (Post).
Useful For: See CHEP/84427 Chimerism-Recipient Engraftment (Post).
Interpretation: See CHEP/84427 Chimerism-Recipient Engraftment (Post).
Reference Values:
An interpretive report will be provided.
Clinical References: See CHEP/84427 Chimerism-Recipient Engraftment (Post).
Chimerism-Recipient Engraftment (Post)
Clinical Information: Patients who have had donor hematopoietic cells infused for the purpose of
engraftment (i.e., bone marrow transplant recipients) may have their blood or bone marrow monitored for
an estimate of the percentage of donor and recipient cells present. This can be done by first identifying
unique features of the donorâ€s and the recipientâ€s DNA prior to transplantation and then
examining the recipientâ€s blood or bone marrow after the transplantation procedure has occurred. The
presence of both donor and recipient cells (chimerism) and the percentage of donor cells are indicators of
transplant success. Short tandem repeat (STR) sequences are used as identity markers. STRs are di-, tri-,
or tetra-nucleotide repeat sequences interspersed throughout the genome at specific sites, similar to gene
loci. There is variability in STR length among people and the STR lengths remain stable throughout life,
making them useful as identity markers. PCR is used to amplify selected STR regions from germline
DNA of both donor and recipient. The lengths of the amplified fragment are evaluated for differences
(informative markers). Following allogeneic hematopoietic cell infusion, the recipient blood or bone
marrow can again be evaluated for the informative STR regions to identify chimerism and estimate the
proportions of donor and recipient cells in the specimen.
Useful For: Determining the relative amounts of donor and recipient cells in a specimen An indicator
of bone marrow transplant success
Interpretation: An interpretive report is provided, which includes whether chimerism is detected or
not and, if detected, the approximate percentage of donor and recipient cells. It is most useful to observe a
trend in chimerism levels. Clinically critical results should be confirmed with 1 or more subsequent
specimens.
Reference Values:
An interpretive report will be provided.
Clinical References: Antin JH, Childs R, Filipovich AH, et al: Establishment of complete and mixed
Current as of January 4, 2013 7:15 pm CST 800-533-1710 or 507-266-5700 or MayoMedicalLaboratories.com Page 439