Sorted By Test Name - Mayo Medical Laboratories

HIVFA
81758
FHIVD
91901
Clinical References: 1. Constantine N: HIV antibody assays May 2006. In HIV InSite Knowledge
Base (online textbook). Available at URL: http://hivinsite.ucsf.edu/InSite?page=kb-00&doc=kb-02-02-01
2. Centers for Disease Control and Prevention: Revised recommendations for HIV testing of adults,
adolescents, and pregnant women in health-care settings. MMWR Morb Mortal Wkly Rep
2006;55(RR14):1-17 3. Hariri S, McKenna MT: Epidemiology of human immunodeficiency virus in the
United States. Clin Microbiol Rev 2007;20:478-488 4. Owen SM, Yang C, Spira T, et al: Alternative
algorithms for human immunodeficiency virus infection diagnosis using tests that are licensed in the
United States. J Clin Microbiol 2008;46:1588-1595
HIV-1 Antibody Confirmation by Immunofluorescence, Serum
Clinical Information: HIV type 1 (HIV-1) is the major etiologic agent of AIDS. The virus is
transmitted through sexual contact, by exposure to blood (including sharing contaminated needles and
syringes) or certain blood products, or from an infected mother to her fetus or child during the perinatal
period. HIV-1 has been isolated from patients with AIDS or AIDS-related complex and from healthy
persons at high risk for AIDS. Although there are 2 types of AIDS-causing viruses, namely HIV-1 and
HIV-2, infections with HIV-2 outside of West Africa are uncommon. This assay can be used to confirm
the presence of HIV-1-specific antibodies when the initial screening assay is repeatedly reactive. See HIV
Serologic Screening Algorithm (excludes HIV rapid testing), and HIV Rapid Serology Follow-up
Algorithm in Special Instructions.
Useful For: Confirmatory detection of HIV-1 antibodies in human serum specimens that are repeatedly
reactive by antibody screening assays or showed indeterminate or uninterpretable results by HIV-1
antibody Western blot
Interpretation: A negative result indicates the absence of HIV-1 specific antibodies. A positive result
indicates the presence of HIV-1 specific antibodies. An indeterminate result does not imply that HIV-1
antibodies are present or absent in the serum specimen. Rather, it indicates that HIV-1 antibody status of
the specimen cannot be resolved through the use of this assay. The correct approach in such situations
should be based on subsequent repeat testing and the patient's clinical findings. See HIV Serologic
Screening Algorithm (excludes HIV rapid testing) and HIV Rapid Serology Follow-up Algorithm in
Special Instructions.
Reference Values:
Negative
See HIV Serologic Interpretive Guide in Special Instructions for further interpretive information.
Clinical References: 1. Sullivan MT, Mucke H, Kadey SD, et al: Evaluation of an indirect
immunofluorescence assay for confirmation of human immunodeficiency virus type 1 antibody in U.S.
blood donor sera. J Clin Microbiol 1992;30:2509-2510 2. Branson BM, Handsfield HH, Lampe MA, et al:
Centers for Disease Control and Prevention: Revised recommendations for HIV testing of adults,
adolescents, and pregnant women in health-care settings. MMWR Recomm Rep 2006;55;1-17 3. Hariri S,
McKenna MT: Epidemiology of human immunodeficiency virus in the United States. Clini Microbiol
Rev 2007;20:478-488
HIV-1 Direct Antigen (Non-ICD)
Reference Values:
Reference Range: Negative
The HIV p24 antigen detected by this method has not had
the immune complexes dissociated; therefore, the antigen
value represents the non-complexed p24 antigen level.
The measurement of the p24 (core) antigen has been
shown to be useful in detecting circulating, replicating
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