A Guide to the Russian Academy of Sciences - University of Texas ...

We have the current support from Siberian Branch of the Russian Academy
of Sciences project "Physical and chemical bases of living system evolution"
and Russian Human Genome Project.
Subjects of Scientific Collaboration:
Molecular genetic mechanisms of evolution and fixation of Canidae
specific satellite-like repeats in the genomes of the modern Canidae
species (Romashchenko Aida G., Ph.D.).
The study of genetic consequences of the radiation pollution in the
population-family samples from residents of Altai territories: the
structural and functional variability of M-CSF receptor gene and mtDNA
(Romashchenko A.G., Ph.D., Voevoda Mikhail I., Ph.D.).
Analysis of the mitochondrial and nuclear genes DNA polymorphism in
populations of the native inhabitants of North-East Asia (Voevoda
Mikhail I., Ph.D.).
Molecular evolution of the regulatory regions in the genomes of the dog
family (Canidae). (Kolesnikov Nikolai N., Dr.Sci.).
Synthesis of polydeoxyribonucleotides for project needs and development
of methods of macroscale super-rapid synthesis of oligonucleotides
(Kobsev Viktor F., Ph.D.).
3. Laboratory of Gene Expression Control.
Staff:
Tatyana I. Merkulova, Ph.D., Head of the Laboratory
Vasily M. Merkulov, Ph.D.
Rimma L. Mitina, Ph.D.
Galina A. Kovalenko, Ph.D.
Soya B. Levashova
Gennady V. Vasiliev
Yaroslav R. Efremov
Subjects of Research:
Molecular mechanisms of gene expression control.
Endogenic nucleases in antiviral defence.
General Results 1993-1995:
1) Glucocorticoid responsive elements (GREs) located -252 to -209 bp
upstream and +1011 to +1054 bp downstream of the transcription
initiation site of the mouse metallotionein-I (mMT-I) gene were
identified in transient transfection experiments. However, the
promoter region of the mMT-I gene (-330 to +70 bp) was found to
provide low, if any, glucocorticoid induction of the linked CAT
gene, while showing strong cadmium regulation, comparable with the
in vivo level.
In studies of highly purified GR binding to EcoR I fragments of rTO
gene DNA covering the region of localization of this gene from -8kb
to +8kb , we have found a new region of specific binding to GR
extending from the end of the 4th intron to the exon G. In determined
nucleotide sequence of this region, with the help of computer
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