A Guide to the Russian Academy of Sciences - University of Texas ...

Marina V. Lavrentyeva
Elena M. Kaftanovskaya, Ph.D.
Sergey B. Kuznetsov
Subjects of Research:
Study of cells differentiation and their reversibility in mammals.
Comparative mapping of mammalian genomes.
General Results 1993-1995:
Cell hybrids between embrionic stem cells and mouse Peucocytes were
obtained during differentiation for study of organization and
inactivation mechanism of mammilian X-chromosome. Using them
the ability of a differentiated cell to dedifferentiate till the
pluripotential state while bringing it into an embryonal stem cell was
demonstrated for the first time. It is shown that a short-term rize of
DNA one-chain breaks and sister chromatid exchanges can be seen in
2-3 miotic divisions after the beginning of spontaneous
differentiation of embryonal stem cell in vitro as well as at
differentiation introduction in teratocarcynoma cells.
At normal preimplantational mouse development the level of DNA
breaks is rizing at the 8-16 blastomeres stage. Works on obtaining
transgenic animals in a carnivora representative American mink are
carried out.
Using cell hybrids, by methods of electrophoretic division of proteins
and Sauthern blot hybridization about 80 genes on an American mink
chromosome, about 20 genes on a common shrew chromosome and 4
genes on a domestic pig chromosome are assigned.
A collection of cell lines, containing one chromosome of pig 2, 5, 8, 12
or t(1, 13) against the background of mink chromosomes was
obtained as well as a collection of human monochromosomal hybrid
lines with chromosomes 3, 5, 12, 13, 15, 18, 21 and X. These lines
are a valuable source of precise physical mapping of mammal
chromosomes and obtaining chromosome-specific libraries.
Participation in State and International Programs:
Human Genome
RFBR
New Trends in Genetics
The wellcome trust 039045/Z/93/Z/MjM/LEC
NATO-project (HTECH. Zf 931407)
Subjects of Scientific Collaboration:
University Halle, Germany
University of York, UK
Royal University, Copenhagen, Denmark
3. Laboratory of Evolutionary Genetics.
Staff:
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