Biochemische Charakterisierung der siRNA-vermittelten Erkennung ...

3 Material
3.8.4 Primer
Name
Ago2 fwd AII
Ago2 rev AgeI
Ago2 rev AgeI Stopp
pET41b fwd
pET41b BlpI rev
Sequenz (5' → 3')
TGA CTA GCT TAA GAT GTA CTC GGG AGC CGG CC
TCA GTC AAC CGG TAG CAA AGT ACA TGG TGC GCA GAG
TCA GTC AAC CGG TTC AAG CAA AGT ACA TGG TGC GCA GAG
CCA GCA ACC GCA CCT GTG
GGG TTA TGC TAG TTA TTG CTC AGC
972 rev AGA GGG TTA GAG CAG CCT T
1233 fwd GGC AGA TGA AGA GGA AGT AC
1411 fwd GGA GCA GAA ACA CAC CTA C
1506 rev CTA GCA GTC GCT CTG ATC A
2316 fwd ACC GGC AGG AGA TCA TAC A
2519 rev GCA CCA CGA TGA AGG TGA T
pET41b rev T7 term
TGC TAG TTA TTG CTC AGC GGT
3.9 Kits
Name
BigDye R○ Terminator v3.1 Cycle Sequencing Kit
Bradford Assay Kit
ECL TM Western Blotting Reagent
GenElute Plasmid Miniprep Kit
Non-Interfering TM Protein Assay
Nucleobond Xtra Maxi Kit
Phusion High Fidelity Polymerase Kit
Silver Stain Kit
Wizard R○ SV Gel and PCR Clean-Up Kit
Hersteller
Applied Biosystems
Biorad
Thermo Fisher Scientic
Sigma-Aldrich
Biosciences
Macherey & Nagel
Finnzymes
Biorad
Promega
48