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extent <strong>of</strong> coagulation, reduction <strong>of</strong> litmus, acid production,<br />

<strong>and</strong> peptonization. A table shows the results.<br />

“Soybean <strong>milk</strong> serves as a excellent culture medium for<br />

the propagation <strong>of</strong> the organisms studied.” After 48 hours<br />

the pH for <strong>soy</strong>bean <strong>milk</strong> was lower (more acidic) than that<br />

for cow’s <strong>milk</strong> when cultured with the following organisms:<br />

L. bulgaricus (3.85 vs. 4.60 at 37ºC), S. lactis (4.13 vs. 4.30<br />

at 21ºC), A. aerogenes (5.0 vs. 5.10 at 37ºC), L. <strong>acidophilus</strong><br />

(3.60 vs. 4.10 at 37ºC). The cell size <strong>of</strong> L. <strong>acidophilus</strong> was<br />

larger in <strong>soy</strong>bean <strong>milk</strong> than in cow’s <strong>milk</strong>. “Growth <strong>of</strong> L.<br />

bulgaricus in <strong>soy</strong>bean litmus <strong>milk</strong> showed that coagulation<br />

occurred more rapidly, whereas in litmus <strong>milk</strong> acid<br />

production <strong>and</strong> litmus reduction were more pronounced.”<br />

Address: Dep. <strong>of</strong> Bacteriology, The Ohio State Univ.,<br />

Columbus, Ohio.<br />

175. Gehrke, Charles W.; Weiser, Harry H. 1948.<br />

A comparative study <strong>of</strong> the biochemical activity <strong>of</strong><br />

Streptococcus lactis, S. citrovorus, <strong>and</strong> S. paracitrovorus<br />

when grown in cow’s <strong>milk</strong> <strong>and</strong> <strong>soy</strong>bean <strong>milk</strong>. J. <strong>of</strong> Dairy<br />

Science 31(4):213-22. April. [17 ref]<br />

• Summary: These three microorganisms are widely used<br />

to culture butter. “Soybean or vegetable <strong>milk</strong> is used<br />

extensively throughout Japan <strong>and</strong> China for infant feeding as<br />

well as for adults.” The introduction <strong>of</strong> <strong>soy</strong>bean <strong>milk</strong> to the<br />

American people has occurred only recently. Soybean <strong>milk</strong><br />

has been manufactured in the form <strong>of</strong> a powder. It has been<br />

used with good results... especially in those countries that<br />

fi nd it cheaper to use a vegetable <strong>milk</strong>... The development by<br />

butter organisms <strong>of</strong> diacetyl [which is partially responsible<br />

for the aroma <strong>of</strong> butter] <strong>and</strong> related compounds in vegetable<br />

<strong>milk</strong> <strong>and</strong> products made from it may be desirable from a<br />

commercial st<strong>and</strong>point.”<br />

The butter cultures were obtained from the Department<br />

<strong>of</strong> Dairy Industry, Iowa State College, Ames. The<br />

<strong>soy</strong>bean <strong>milk</strong> was obtained from Harry Miller, Director<br />

<strong>of</strong> the International Nutrition Laboratory, Mt. Vernon,<br />

Ohio. Cow’s <strong>milk</strong> <strong>and</strong> <strong>soy</strong>bean <strong>milk</strong> were inoculated<br />

<strong>and</strong> incubated, then measurements <strong>of</strong> the following were<br />

taken at regular intervals up to 216 hours: pH, titratable<br />

acidity, volatile acidity, <strong>and</strong> Mg Ni salt. The production <strong>of</strong><br />

acetylmethylcarbinol (AMC) <strong>and</strong> diacetyl in <strong>soy</strong>bean <strong>milk</strong><br />

by butter culture organisms was not evident until after an<br />

incubation period <strong>of</strong> 48 hours; however, upon incubation <strong>of</strong><br />

cultured <strong>soy</strong>bean <strong>milk</strong> for 168 to 216 hours, larger amounts<br />

<strong>of</strong> the fl avor <strong>and</strong> aroma compounds were produced in<br />

<strong>soy</strong>bean <strong>milk</strong> than in cow’s <strong>milk</strong>. “The values obtained for<br />

the titratable acidity in cow’s <strong>milk</strong> <strong>and</strong> cow’s <strong>milk</strong> to which<br />

0.15 per cent citric acid had been added were nearly twice as<br />

great as those secured for the <strong>soy</strong>bean <strong>milk</strong>. Address: Dep. <strong>of</strong><br />

Bacteriology, The Ohio State Univ., Columbus, Ohio.<br />

176. Fairchild, David. 1948. Early experiences with the<br />

<strong>soy</strong>bean. Soybean Digest. Nov. p. 14-15. [2 ref]<br />

HISTORY OF SOY YOGURT & CULTURED SOYMILK 93<br />

© Copyright Soyinfo <strong>Center</strong> 2012<br />

• Summary: “It was not until 1897 that I fi rst saw <strong>soy</strong>beans<br />

growing... I found my friend Merton B. Waite had been<br />

trying to grow <strong>soy</strong>beans on his farm outside Washington<br />

[DC, in Maryl<strong>and</strong>; see Fairchild, Oct. 1948], but with little<br />

success. They had been sent in by some American consul<br />

or missionary, I believe. At about the same time Dr. George<br />

T. Moore [who by 1903 was Physiologist in Charge <strong>of</strong> the<br />

Lab. <strong>of</strong> Plant Physiology, Bureau <strong>of</strong> Plant Industry, USDA,<br />

working on <strong>soy</strong>bean root bacteria] in working on the root<br />

nodules <strong>of</strong> leguminous plants had discovered that the nodules<br />

contained bacteria. Waite <strong>and</strong> I talked over the matter <strong>of</strong> the<br />

failure <strong>of</strong> his <strong>soy</strong>beans <strong>and</strong> wondered if they might require<br />

special bacteria, so I wrote out to Japan <strong>and</strong> imported several<br />

pounds <strong>of</strong> soil from a <strong>soy</strong>bean fi eld.<br />

“We made a little experiment, planting alternate rows <strong>of</strong><br />

<strong>soy</strong>beans with <strong>and</strong> without the addition <strong>of</strong> this Tokyo soil.<br />

The effect <strong>of</strong> the imported soil was immediately apparent as<br />

the plants grown in it made a far better growth <strong>and</strong> had their<br />

roots covered with bacterial nodules, whereas the control<br />

were practically without any.<br />

“Photographs had yet to come into any general use as<br />

records <strong>of</strong> agricultural experiments, but Waite had taken up<br />

photography as a hobby <strong>and</strong> made excellent photographs<br />

with his st<strong>and</strong> camera. So at harvest time he pulled up an<br />

equal number <strong>of</strong> <strong>soy</strong>bean plants from the soil-treated rows<br />

<strong>and</strong> from the controls, <strong>and</strong> the only photographic record<br />

in existence <strong>of</strong> this little experiment (the fi rst <strong>of</strong> its kind, I<br />

suppose, in the world) is this negative taken by Waite in the<br />

autumn <strong>of</strong> 1897.<br />

“Although these experiments did not at the time lead<br />

to more extensive trials, they indicate the awakening <strong>of</strong> our<br />

interest in <strong>soy</strong>beans.<br />

“In that fall <strong>of</strong> 1897 it was my privilege to organize the<br />

<strong>of</strong>fi ce <strong>of</strong> plant introduction in the Department <strong>of</strong> Agriculture.<br />

We began introducing a great number <strong>of</strong> different kinds <strong>of</strong><br />

plants, among them, as I see from our inventories printed<br />

at the time, occasional small collections <strong>of</strong> <strong>soy</strong>beans from<br />

China <strong>and</strong> Japan. They were obtained mostly through<br />

correspondence with missionaries <strong>and</strong> consuls stationed<br />

there.<br />

“At that time the Department had no testing ground near<br />

Washington where we could grow miscellaneous vegetables,<br />

including these <strong>soy</strong>bean collections. It was not until several<br />

years had passed that facilities were provided on the socalled<br />

‘Potomac Flats’ [in Washington, DC] <strong>and</strong> James H.<br />

Beattie, an enthusiastic young horticulturist, took over the<br />

planting <strong>of</strong> our introduced seeds.<br />

“The <strong>soy</strong>beans did well <strong>and</strong> Beattie soon had on his<br />

h<strong>and</strong>s a quantity <strong>of</strong> seeds. But we didn’t know just what to<br />

do with these strange beans. When cooked in the way <strong>other</strong><br />

beans were prepared they had a strange fl avor that nobody<br />

seemed to like...<br />

“It was not, I think, until the <strong>of</strong>fi ce <strong>of</strong> forage crop<br />

investigations was organized <strong>and</strong> C.V. Piper took charge

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