Abstracts Book - IMRC 2018

• SB5-O016
FOCUSED ELECTRON AND ION BEAM (FEB-FIB) MODIFICATION OF
BIOCERAMIC SURFACES FOR OSTEOBLAST CELLS ADHESION
OPTIMIZATION
Feray Bakan 1 , Melike Çokol Çakmak 2 , Meltem Sezen 1 , Elif Celik 2 , Zaeema Khan 2 , Tolga Sutlu 1
1 Sabanci University, SUNUM, Turkey. 2 Sabanci University, FENS, Turkey.
As the calcified tissues of mammalians consist of calcium phosphate (CP);
compounds of synthetic CPs are widely used in orthopedic implants for several
targets such as; repairment and reconstruction of bone tissue defects, bone
fracture healing, strengthening dental tissues and as coatings in implant
materials owing to their excellent biocompatibility and bioactivity. The biological
interface between an orthopedic implant and the surrounding host tissue may
have a significant effect on the clinical outcomes. Desired effects include bony
ingrowth (osseointegration), stimulation of osteogenesis (osteoinduction),
increased vascularization, and improved mechanical stability. Among these
factors, the surface texture or micro-topography of an implant material can
drastically affect the implant-cell interactions. Therefore, examining cell
interactions by forming controlled surface textures has crucial importance.
For this purpose, the surface topography of two calcium phosphate bioceramics;
hydroxyapatite (HA) and biphasic calcium phosphate (BCP) were modified in
dual-beam platforms (FIB-SEM) and the cell interaction of mammalian
osteoblast-like cells (MG-63) on the related surfaces was investigated in vitro.
The crucial point is performing the corresponding processes inside FIB-SEM
systems via direct ion and electron nano-processing and therefore carrying out
the modifications in a variety of sizes micron to nanometer and in different
geometries which are local and selective. Cell-surface interactions were then
observed both using Fluorescence Microscopy and SEM imaging. Besides,
Fluorescence microscopy images were analyzed by using ImageJ software to
obtain qualitative information on cell adhesion behavior on functionalized
surfaces of CP based bioceramic materials.
Acknowledgment: The authors would like to thank TUBITAK 115M788 Project
for financial support.
Keywords: Surface Functionalization, Bioceramics, Ion-Beam Lithography
Presenting authors email: feraybakan@sabanciuniv.edu