Abstracts Book - IMRC 2018

• SB6-P053
DESIGN AND IN VITRO EVALUATION OF A BIOINSPIRED TRI-
BIOPOLYMER HYDROGEL
Jorge Alfonso Tavares Negrete 1 , Diana Karime Navarro Arias 1 , Hugo Oñate Robledo 1 , Argelia
Rosillo de la Torre 1 , Jorge Delgado Garcia 1 , Laura Edith Castellano Torres 1 , Irais Amaranta
Quintero Ortega 1
1 Universidad de Guanajuato campus León, Chemical Electronic Biomedical Engineering,
Mexico.
Cartilage extracellular matrix (CECM) is a natural scaffold composed mainly of
water, collagen, and glycoproteoglycans that are secreted by chondrocytes.
Daily activities such as mechanical overload, bad posture, incorrect sport
performance and age promotes its degeneration. Chondrocytes death is the
inherent consequence of the (CECM) degradation due to mechanical pressure.
Therefore, if the specialized cells have not the ability to replicate they will be
diminished, and when a critical point is reached the existing chondrocytes will
be unable to synthesize enough CECM that allow them to survive and they will
gradually disappear. Usually hydrogels formed by chitosan and other natural
polymers such as collagen or hyaluronic acid are used to mimic the CECM and
encourages its regeneration. A three polymer crosslinked hydrogel was
synthesized with chitosan as the main chain, which will provide biocompatibility,
antibacterial properties and structural strength; collagen was incorporated for
enhancing the flexibility and tensile strength and hyaluronic acid (a
glycoproteoglycan) was added for promoting cellular adhesion in the native
cartilage, as a source of nutrient for chondrocytes and collagen precursor. The
synthesis of this biomaterial was done by the “Schiff” base and acetylation
reaction, which take advantage of the amino and hydroxyl groups of the
polymers, allowing crosslinking, which results in a biomaterial that mimic the
physical and biological properties of the cartilage. The hydrogel was
physicochemical characterized by a swelling behavior study, Fourier Transform
Infrared Spectrometry, Scanning Electronic Microscopy; and shear and stress
resistance was measured. The gelation kinetics was studied by reometry and by
UV-vis spectrophotometry. The crosslinked percentage was determined by
ninhydrin assay. In vitro degradation evaluation was performed using
collagenase and the immune response was studied with rat immortalized
macrophages.