Abstracts Book - IMRC 2018

• SB6-P031
EFFECT OF GOLD NANOPARTICLES FUNCTIONALIZED WITH
CALRETICULIN ON THE EXPRESSION OF GROWTH FACTOR
Sara Paola Hernández 1 , Diana Ginette Zarate Trivi�o 1 , Moisés Franco Molina 1 , Juan José
Bollain y Goytia de la Rosa 2 , Cristina Rodríguez Padilla 1
1 Universidad Autónoma de Nuevo León, Immunology, Mexico. 2 Universidad Autónoma de
Zacatecas, Immunology, Mexico.
Currently, the search for materials which can help wound healing in diabetic foot
ulcers is increasing. Gold nanoparticles (GNPs) are one of the most studied
materials; they have a very high surface area, so they can be easily functionalized
and bioconjugated with proteins. In wound healing, it has been shown that several
chaperone proteins are involved. Calreticulin (CRT), is an endoplasmic reticulum
chaperone protein involved in production of growth factors such as Transforming
Growth Factor beta (TGF-β 1) and Vascular Endothelial Growth Factor (VEGF), wich
are involved in tissue remodeling and wound healing. The objective of this work was
to determine the effect of GNP´s functionalized with CRT in the expression of TGFβ
1 and VEGF. GNPs were synthetized via chitosan reduction reaction according to
Turkevich method. GNPs were functionalized with CRT and characterized by means
of UV–vis and dynamic light scattering (DLS). Mice were injected with streptozotocin
to induce diabetes and the blood glucose levels were measured for 30 days. A
wound model was made in the mice and the wound area was determined. Tissues
were evaluated by immunohistochemistry to determine the expression of growth
factors. Data were statistically analyzed analyzed by t-Student and ANOVA in the
statistical program GraphPrism version 7 with a p≤0.05. Nonfunctionalized GNPs
presented a surface plasmon resonance (SPR) peak at 524 nm and absorbance of
0.228 in contrast with functionalized GNPs that presented a SPR peak at 525 nm
and absorbance of 0.174. The average size of nonfunctionalized GNPs were 5.7 ±
1.07 and exhibited a polydispersity index (PDI) value of 0.3, indicating a narrow
particle size distribution, unlike functionalized GNPs (92.39 ± 0.94 nm) that
presented PDI = 0.5. Diabetic mice had blood glucose levels above 160 mg/dL in
contrast with the healthy group (90 mg/dL). Mice treated with functionalized GNPs
healed faster in both groups, healthy and diabetics. The expression of TGF-β 1 and
VEGF increased in mice treated with functionalized GNPs in both groups, healthy
and diabetics. The GNPs synthesis using chitosan as a reducing agent allowed
functionalization of the nanoparticles with calreticulin. For diabetic mice, wounds
treated with GNPs functionalized with calreticulin, healed faster.
Keywords: Biomaterials, Gold nanoparticle, Wound healing
Presenting authors email: s_2044@hotmail.com