Abstracts Book - IMRC 2018

• SB6-O007 Invited Talk
SALIVARY PROTEINS AND THEIR POTENTIAL TO REDUCE
BACTERIAL COLONIZATION ON IMPLANT SURFACES
Miryam Martínez Hernández 1 , Victor I. García Pérez 1 , René Olivares Navarrete 2 , Argelia
Almaguer Flores 1
1 Universidad Nacional Autónoma de México, Facultad de Odontología, DEPeI, Mexico. 2 Virginia
Commonwealth University, Biomedical Engineering, School of Engineering., United States.
Biofilm formation on biomedical surfaces such as titanium (Ti) dental implants
is a persistent problem that can cause implant failure. Bacterial colonization is
the first step in the biofilm formation on a surface; therefore, the prevention of
bacterial adhesion represents a natural strategy to avoid device-related biofilm
infections. Once exposed to the oral cavity, dental implants are immediately
covered by a thin pellicle mainly composed by the proteins present in the saliva
and blood. The physico-chemical properties of the implant surface, strongly
influence the characteristics of the protein pellicle formed on it; affecting the
performance and longevity of the implanted device.
In our research group, we have been studying the patterns of salivary protein
adsorption on titanium surfaces with different roughness and wettability. The
proteomic analysis performed by Nano-LC-MS/MS (nano mass spectrometry)
revealed that from the total of the salivary proteins detected adsorbed on the Ti
surfaces, 95.6 % of them have not been previously identified on the dental
enamel. This finding confirmed the notion of the high selectivity for the protein
pellicle formation on a surface, and the influence of the surface characteristics
in the protein adsorption process.
We have tested how the previous adsorption of histatin 5, cystatin S and α-
amylase, on Ti implant surfaces, affects the colonization of three important oral
species; Streptococcus gordonii, Fusobacterium nucleatum, and
Porphyromonas gingivalis. Our results showed that the colonization of each
bacterial strain was selectively reduced depending the salivary protein tested.
This information could be useful in the development of new strategies for
surface functionalization, based on the capacity of the salivary proteins to
reduce bacterial colonization.
Keywords: Titanium dental implants, Biofilm infections, Salivary proteins
Presenting authors email: aalmaguer@comunidad.unam.com