Abstracts Book - IMRC 2018

• SB2-P017
UNILATERAL ARTHRITIS MODEL IN VIVO FOR THE EVALUATION
OF A FUSION ANTIBODY
Elvia Zárate 1,2
1 Unidad Académica Multidisciplinaria Reynosa-Aztlán, Pharmacology, Mexico. 2 Universidad
Autónoma de San Luis Potosí, Pharmacology, Mexico.
The Etanercept is a recombinat protein neutralizing of TNF-α. This
biomolecules will lose their patent in the next years, opening the possibility to
be developed by other companies. biosimilar is the replicate version of an
already authorized biological medicinal product with demonstrated similarity in
physicochemical characteristics, efficacy and safety, based on a comprehensive
comparability exercise.
Rodent models of arthritis constitute a spectrum on different aspects of the
disease. However, there is no relevant animal model to evaluate the efficacy of
drugs designed for RA.
The model of unilateral arthritis in male ICR mice represents an alternative for
the evaluation of therapeutic proteins related to arthritic processes, since it
allows the evaluation of pain behaviors and functional disability, factors that
represent the highest degree of involvement in people living with arthritis
The unilateral arthritis model is based on four weekly immunizations with
complete Freunds adjuvant in the right knee of the mouse, to create and sustain
a pro-inflammatory environment similar to that of patients with arthritis. The
model evaluates pain behaviors (shaking, inflammation and proinflammatory
markers) and functional disability (guarding the limb, number of uprisings and
loss of use of the limb); These behaviors are evaluated prior to the first
immunization with CFA and then followed up for a month. The administration
of ETN, test and reference, was performed three times intra-peritonially (25 mcg
/ Kg of weight).
For this work there were 5 evaluation groups: 1) white (group without arthritic
status and did not receive medication, 2) without AR status and receiving
placebo, 3) with arthritic status and receiving placebo, 4) with arthritic status and
receiving ETN of reference and 5) with arthritic state and receives ETN test.