Abstracts Book - IMRC 2018

• SB6-P025
STUDY OF BIOACTIVITY AND ANTIBACTERIAL PROPERTIES OF
SILVER-DOPED HYDROXYAPATITE.
Jhan Danilo Salazar Martínez 1 , Pedro Jose Arango Arango 1 , Belarmino Segura Giraldo 1 ,
Elisabeth Restrepo Parra 1
1 Universidad Nacional de Colombia, Ciencias Exactas y Naturales, Colombia.
In this Project, we performed a study of bioactivity and antibacterial properties
of silver-doped synthetic hydroxyapatite, and silver-doped biogenic
hydroxyapatite. Synthetic hydroxyapatite powders were prepared using the
chemical precipitate method, and biogenic hydroxyapatite powders were
obtained pig bone by means of the calcination method. Obtained powders were
doped with silver nanoparticles and then were characterized using x-ray
diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR),
scanning electron microscopy (SEM), and x-ray dispersive energy (EDX). For XRD
analysis, the planes diffracted in the samples were (002), (210), (211), (112), (300)
and (202) corresponding to HAp, and (111) and (200) corresponding to silver
metal. FTIR spectra show the vibrational bands associated with both hydroxyl
functional group (OH - ) and phosphate functional group (PO4 3- ) present on HAp.
For bioactivity and antibacterial analysis, powders were compacted into pellets
of 13mm diameter applying a pressure of 100MPa. The bioactivity test was
carried out following the protocol described by Kokubo and Takadama; the
samples were immersed in simulated body fluid (SBF) at 37°C for a period of 3
days, 7 days, and 14 days. After the bioactivity study, the samples were again
analyzed using Fourier transform spectroscopy, scanning electron microscopy
and x-ray dispersive energy. The antibacterial test of the samples against P.
aeruginosa and P. Putida was carried out to verify the length of diameter of the
inhibition zone; the samples were put on the surface of nutrient agar medium
containing the bacteria and were incubated at 37°C for 24 hours.
Keywords: Hydroxyapatite, Bioactivity, Antibacterial properties
Presenting authors email: jhdsalazarma@unal.edu.co