Abstracts Book - IMRC 2018

• SB2-P013
MAGNETO-LUMINESCENT GAG:CE NANOPARTICLES
FUNCTIONALIZED WITH FOLIC ACID FOR BIOIMAGING OF BREAST
CANCER CELLS
Carlos Muñoz Bustos 1 , Akhil Jain 2 , Gustavo Alonso Hirata Flores 2 , Karla Oyuky Juárez Moreno 3,4
1 Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Physical
chemistry, Mexico. 2 Centro de Nanociencias y Nanotecnología CNyN-UNAM, physical
chemistry, Mexico. 3 Centro de Nanociencias y Nanotecnología CNyN-UNAM,
Nanobiotechnology, Mexico. 4 Consejo Nacional de Ciencia y Tecnologia - CONACYT,
Nanobiotechnology, Mexico.
The luminescent and magnetic properties of gadolinium aluminate garnet
nanoparticles doped with cerium (GAG:Ce) and functionalized with folic acid for
biolabeling cancer cells are described in this work. The results demonstrated
that nanoparticles were successfully synthesized by combustion method with
carbohydrazide as fuel. Physicochemical properties and functionalization of
GAG:Ce nanoparticles were characterized by TEM, XRD, FTIR spectra,
fluorescence spectroscopy and UV-vis studies. The photoluminescence studies
of the GAG:Ce nanoparticles in different synthesis stages showed a blue
excitation at 450 nm and a yellow emission at 550 nm, with an optimal doping
concentration of 2% of cerium. The values of magnetic susceptibility displayed
that GAG:Ce nanoparticles have a paramagnetic behavior, which is very likely to
generate magnetic resonance images (MRI). Additionally, the cytotoxicity of bare
and functionalized GAG:Ce nanoparticles in MDA-MB-231 breast cancer cells
was evaluated by MTT assay. Finally, the internalization of GAG:Ce nanoparticles
functionalized with folic acid was demonstrated by confocal microscopy, which
generated fluorescent images of cancer cells, confirming that they can function
as effective biolabels of breast cancer cells.
Acknowledgment:
We appreciate the financing of the project DGAPA-UNAM No. 111017 and the
technical support of E. Aparicio, I. Gradilla and F. Ruiz
Keywords: GAG:Ce nanoparticles, Folic acid, Biolabels
Presenting authors email: ing_carlos@outlook.com