Abstracts Book - IMRC 2018

• SB6-P079
ASSEMBLY AND PHYSICOCHEMICAL CHARACTERIZATION OF
LIPOSOMES WITH A BIOACTIVE CONJUGATED LINOLEIC ACID
MIXTURE AS A VEHICLE OF ANTITUMORAL DRUGS
Norma Estela Hernandez Loredo 1 , Said Aranda Espinoza 2 , Carmen Gonzalez 1,3 , Gabriela
Navarro Tovar 1,3
1 Universidad Autónoma de San Luis Potosí, Laboratorio de fisiologia Celular, Mexico.
2 Universidad Autónoma de San Luis Potosí, Instituto de Fisica, Mexico. 3 Universidad Autónoma
de San Luis Potosí, Centro de Investigación en Ciencias de la Salud y Biomedicina, Mexico.
The glioblastoma multiforme (GBM) is the most aggressive malignant tumor in
the central nervous system (CNS). The mean survival period for diagnosed
patients is only 12–14 months after standard treatments. The therapeutic
treatments available for GBM include chemotherapy employing tamoxifen (TXF);
however, drug delivery is not effective or specific to glioma cell, and causes
several adverse effects. Therefore, it is necessary to develop new
pharmaceutical delivery system, such as those offered by the nanotechnology;
for instance, liposomes. A recent study in our research group showed that a
bioactive mixture of isomers of conjugated linoleic acid in a 50:50 ratio of cis-9,
trans-11 octadecadienoic acid and trans-10, cis-12 octadecadienoic acid (CLA),
has a selective antitumoral effect on rat glioma C6 cell. Following this line of
research, the present work achieved to assemble liposomes of CLA and
tamoxifen (LCLA-TXF) by thin-film hydration/ultrasonication (TFH) and
electroformation method (EM). The characterization of liposomes obtained by
TFH showed hydrodynamic diameter values of 99.6 ± 54.9 nm and an average
surface charge of -53.6 ± 3.2 mV. On the other hand, liposomes by EM showed
particle size of 4.31 ± 6.75 μm and after extruding the liposome preparation
through a 100 nm membrane, the hydrodynamic diameter of liposomes
reduced to 100.2 ± 31.9 nm with a surface charge of -34.5 ± 16.1 mV. In addition,
the encapsulation efficiency (%EE) was determined by dialysis method at
pH=7.4, with an initial TXF mass of 9 mg in 1:3:6 ratio of drug, cholesterol and
CLA, respectively. The %EE value was 83.27 ± 5.95 % for TFX in the LCLA-TXF
formulation. Other conducted characterizations to LCLA-TXF were Scanning
Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The
results and comparison of both assembly techniques TFH and EM are relevant
to recognize the physicochemical characteristics of LCLA-TXF. Further studies,
such as the localization of TXF in the LCLA-TXF preparation and the TXF release