Abstracts Book - IMRC 2018

• SB6-P082
FORMATION AND CHARACTERIZATION OF
ETHOSOMES AND CYTOTOXICITY EVALUATION
Jesus Alejandro Campa Rivera 1 , Cesar Rodríguez Beas 1 , Jesús Adriana Soto Guzmán 2 , Eduardo
Larios 3 , Aaron Alberto Martinez Higuera 1 , Ramon Alfonso Iniguez Palomares 1 , Ericka Rodríguez
Leon 4
1 Universidad de Sonora, Departamento de Física, Mexico. 2 Universidad de Sonora,
Departamento de Medicina y Ciencias de la Salud, Mexico. 3 Universidad de Sonora,
Departamento de Ingeniería Química y Metalurgia, Mexico. 4 Universidad de Sonora,
Departamento de Física, Mexico.
Ethosomes are liposomes (vesicles formed by phospholipids) that contain
ethanol molecules as cosurfactant which into the bilayer in the region close to
the polar heads of the phospholipid, which makes the vesicles less rigid allowing
a more efficient trans-epidermal diffusion, making them excellent drug carriers.
In the following work, the process of synthesis and characterization of
ethosomes formed by the zwitterionic phospholipid 1,2-Dioleyl- sn-glycero- 3-
phosphatidylcholine (DOPC) in the presence of the ethanol-water solvent 15:85
v / v is described. Said ethosomes were used to encapsulate root extract of
Rumex hymenosepalus which is abundant in polyphenolic molecules.
Ultracentrifugation and microfiltration were used to recover the loaded
ethosomes with the extract the non-encapsulated extract remaining in the
solvent. The obtained product was characterized by UV-Vis spectroscopy,
Confocal Fluorescence Microscopy, Scanning Electronic Microscopy (SEM) and Z
potential, also, the cytotoxicity of the loaded ethosomes with the Rumex hym.
was assessed in peripheral blood cells. The presence of the extract inside the
ethosomes was confirmed using UV-Vis. In the Confocal Microscopy it was
observed that the ethosomes were spherical, with an average size of 1.2 µm. It
was observed that the ethosomes were stable with and without the Rh using Z
potential (µ). The lethal concentration 50 (CL50 ) of the loaded ethosomes with
the Rumex extract over peripheral blood cells was 107 µg/mL.
Acknowledgment: All authors thank Nanotechnology Postgraduate for access
to facilities and the use of SEM and to the Biomaterials Laboratory for the use
of Confocal Microscopy of the University of Sonora.
Keywords: Ethosomes, Rumex hymenosepalus, SEM
Presenting authors email: Alejandrocampa91@gmail.com