Abstracts Book - IMRC 2018

• SB1-P013
STUDY OF THE PARAMETERS INVOLVED IN THE BIOPROCESS OF
XANTHAN GUM PRODUCTION
Hugo Oñate Robledo 1 , Jorge Adrían Botello Rueda 1 , Jorge Alfonso Tavares Negrete 1 , Argelia
Rosillo de la Torre 1 , Irais Amaranta Quintero Ortega 1
1
Universidad de Guanajuato campus León, Chemical Electronic Biomedical Engineering,
Mexico., Mexico.
Xanthan gum is a polymer obtained xanthomonas campestris. The main
applications of this polymer are in the pharmaceutical and food field. Due to its
great chemical, thermal and rheological stability, anionic polymers such as
xanthan gum are currently used in research applied to the field of biomaterials,
such as the inhibition of chondrocyte apoptosis in the treatment of
osteoarthritis. The biological synthesis of xanthan gum starts with the growth
and maintenance of xanthomonas campestris in a liquid medium.
Subsequently, a purification methodology is developed that includes
mechanical and chemical processes for its isolation and conditioning. Finally, the
polymer product of this biological synthesis is characterized by different physical
techniques that provide structural and rheological information of the
biomaterial. The operational parameters that are modified during the biological
synthesis of xanthan gum are the source and concentration of carbon (xylose,
glucose and starch), the source and concentration of nitrogen (DAP, DAS and
ammonium nitrate) and the mechanical agitation of the chemical reactor. The
operational parameters that are modified during the purification of synthesized
xanthan gum are the chemical components (IPA, ethanol and acetone in
conjunction with NaCl for polymer precipitation). The characterization tests
include IR-FTR to study the chemical structure of the polymer, rheological test
to study the mechanical stability of the biomaterial and DSC to study the glass
transition temperature (Tg) of the polymer and consequently its thermal stability
interval. The optimization of the bioprocess in obtaining xanthan gum through
the modification of the synthesis and purification parameters, provides
information about the most important variables involved in obtaining a
biomaterial with possible medical applications.
Acknowledgment: I want to thank the UGTO project CIIC 169/2018
Keywords: xanthan, gum, parameters
Presenting authors email: hgo.933@gmail.com