Abstracts Book - IMRC 2018

• SB6-P052
CHARACTERIZATION AND EVALUATION OF THE CYTOTOXIC
ACTIVITY OF POLYMERS OBTAINED FROM CHITOSAN AND
EGGSHELL.
Yeidy Viviana Arias Andrade 1 , Angela Patricia Castro Ramírez 2 , Juan Carlos Sepúlveda Arias 1 , luz
Angela Veloza 2
1 Universidad Tecnológica de Pereira, Grupo Infección e Inmunidad, Facultad de Ciencias de la
Salud, Colombia. 2 Universidad Tecnológica de Pereira, Grupo Polifenoles, Facultad de
Tecnologías, Colombia.
Introduction. Chitosan is obtained from the deacetylation of chitin found in the
exoskeleton of crustaceans and in the cell wall of fungi. several chitosan-based
biomaterials such as fibers, hydrogels, films and sponges have been
manufactured. These materials have important properties such as low
cytotoxicity, low immunogenicity, biocompatibility and biodegradability. A wide
variety of chemical modifications to chitosan have been reported. In addition,
chitosan has been mixed with other materials in order to generate biomaterials
that can be used in regenerative medicine.
Aim. To obtain, characterize and evaluate the cytotoxic activity of polymers
composed of chitosan and egg shell.
Methodology. Chitosan from Aspergillus niger mycelium and silkworm pupa was
extracted by treatment with 40% NaOH for 5 hours and 20 minutes, respectively.
Then chitosan was solubilized in concentrated HCl and purified using acetic acid.
Chitosan characterization was performed using FTIR, NMR and TGA analysis.
Additionally, the molecular weight was determined by capillary viscosimetry and
the degree of deacetylation was evaluaed by potentiometric titration.
The eggshell pulverized to a particle size of 25 μm and chitosan were mixed with
glycerin by mechanical stirring for 30 minutes, obtaining films by drying at 40 °C.
Subsequently, the material obtained was characterized by DSC, TGA and SEM.
Also, a measurement of the tensile strength was carried out. The cytotoxicity of
the obtained polymer was evaluated on a normal cell line (HEK 293), using the
MTT method for 24 hours.
Results. Chitosan was extracted and characterized from the two sources
evaluated and polymer films with good homogeneity were generated. The
characterization by SEM showed uniformity in the distribution of the
components of the biomaterial. Areas with porous and compact structure were
identified. The films were not cytotoxic to HEK 293 cells.