Abstracts Book - IMRC 2018

• SB3-P008
DEVELOPMENT A FUNCTIONAL MULTI-FILAMENT SUTURE USING
COMMERCIAL 3D PEN AND GELMA
Josy Anteveli Osajima Furtini 1 , Francisca Pereira de Araujo 1 , Conceição de Maria Vaz Elias 2 ,
Lavinia Ribeiro 3 , Edson Cavalcanti Da Silva Filho 1,4 , Anderson de Oliveira Lobo 2,4 , Michele de
Paula Madeira 4
1 Universidade Federal do Piauí, , Brazil. 2 Universidade Brasil, Department of Biomedical
Engineering, Brazil. 3 Universidade Federal do Piauí, Department of Physics, Brazil.
4 Universidade Federal do Piauí, Materials Engineering, Brazil.
Suture materials are commonly used to facilitate the tissue repair process, and
the use of materials capable of accelerating the healing process is a promising
alternative in tissue engineering. Polycaprolactone (PCL) is a synthetic,
bioabsorbable polymer that has excellent mechanical strength and is therefore
used in the production of suture materials. Considering the need for the
therapeutic action of filaments, coating them with materials that facilitate tissue
repair is a good way to improve their overall performance. Herein, we developed
a new method to produce multi-filaments for tissue engineering applications
based on extrusion of PCL. We first extruded the PCL using a commercial 3D
pen. Next, the extruded multi-filaments were treated using oxygen plasma (10
scm O2, 0,32 mBar ) to incorporate oxygen functional groups at the surface. After
that, the PCL multi-filaments were dipped in a solution containing gelatin
methacryloyl (GelMA, 10% w/v) and 0.5% (w/v) of photoinitiator (Irgacure, 2959).
The developed multi-filaments were photo-crosslinked using UV irradiation
(three minutes irradiation time and radiation intensity 4,3 mW/cm 2 ). The
structural, chemical, and thermal properties of the resulting scaffolds were
characterized with scanning electron microscopy equipped with dispersive
energy spectroscopy, attenuated total reflectance Fourier transform infrared
spectroscopy, thermogravimetry, differential scanning calorimetry. Tension
tests were also used to investigate the mechanical properties of filaments. Thin,
homogeneous coatings were obtained, with the main peaks centered at 3600
cm -1 and 3200 cm -1 (related to PCL and GelMA, respectively) and thermal analysis
identified a blend formation between PCL and GelMA. The produced multifilaments
showed a tension similar to industrially-developed, multi-filament
sutures, but with a more favorable tissue compatibility due to their chemical
differences.
Acknowledgment: