Abstracts Book - IMRC 2018

• SB6-P017
SYNTHESIS OF 3D CYLINDERS OF PLA-G-(N-VCL/GLyMA) BY
GAMMA-RAYS AND COVALENT IMMOBILIZATION OF LYSOZYME
TO OBTAIN ANTIMICROBIAL ACTIVITY
G. G. Flores Rojas 1 , F. Lopez Saucedo 1 , V. H. Pino Ramos 1 , N. Dionisio Chávez 1 , C. Alvarez
Lorenzo 2 , A. Concheiro 2 , E. Bucio 1
1 Instituto de Ciencias Nucleares UNAM, Quimica de radiaciones en macromoleculas, Mexico.
2 Universidad de Santiago de Compostela, Departamento de Farmacología, Farmacia y
Tecnología Farmacéutica, Spain.
The goal of the present work was the obtaining of 3D cylinders of poly(lactic acid)
(PLA) with antimicrobial activity. The process to develop these cylinders was
throughout the formation of 3D cylinder by 3D print then the cylinders were
grafted with N-vinyl caprolactam (N-VCL) and glycerol methacrilate (GlyMA) by
two step method, using different monomer concentrations as well as absorbed
doses. The grafted N-VCL provided thermal responsiveness while the GlyMA
supplied reactive groups by means of its hydroxyl groups. The new materials
were chemically modified with sodium periodate to provide formyl groups
which were used to carry out the covalent immobilization of lysozyme. The
antimicrobial activity was tested using as standard bacteria Micrococcus
Lysodeikticus at different pH values, temperatures, and activity cycles.
Acknowledgment:
This work was supported by DGAPA-UNAM under Grant IN201617 (Mexico);
MINECO under Grant SAF2014-52632-R (Spain); FEDER. The authors thank B.
Leal ICN-UNAM for technical assistance.
Keywords: Poly(lactic acid) (PLA), Lysozyme, Gamma-rays
Presenting authors email: ggabofo@hotmail.com