Abstracts Book - IMRC 2018

• SB2-O024
FIBROUS MATS BASED ON BIOPOLYESTERS AND
HYDROXYAPATITE MANUFACTURED BY CENTRIFUGAL SPINNING
(FORCESPINNING ® ) WITH POTENTIAL USE AS BONE SCAFFOLDS
Victoria Maria Padilla Gainza 1 , Heriberto Rodríguez Tobías 1 , Graciela Morales 1 , Karen Lozano 2 ,
Vanessa Montaño Machado 3 , Diego Mantovani 3
1
Centro de Investigación en Química Aplicada - CIQA , Síntesis, Mexico. 2 University of Texas Rio
Grande Valley, Mecanica, United States. 3 Université Laval, Min-Met-Materials Eng. & CHU,
Canada.
Hydroxyapatite (n-Hap) is a well-recognized biomaterial with regenerative
characteristics, thus positioning as suitable candidate for bone restoration. The
development of polymeric composites containing n-Hap has been extensively
explored due to their synergistic mechanical performance, standing out in
recent years the use of synthetic and natural biopolymers. This study deals with
the production of fibrous materials based on poly(D,L-lactic acid) (PDLLA) and
poly(3-hydroxybutyrate) (PHB) with n-Hap developed through a centrifugal
spinning technique, (Forcespinning®). In this sense, variables related to the
concentration of n-Hap (5, 10 and 15 wt %) and operational conditions (spinneret
angular rate) were considered to evaluate their influence on the final
morphology and fiber average diameter. The influence of n-Hap on the
morphological, thermal and mechanical performance of optimized mats was
evaluated. For the PDLLA systems, the results showed that the appearance of
beads and beaded fiber is promoted at higher n-Hap concentration. On the
contrary, for the PHB systems, homogeneous fibers were obtained in the entire
interval of n-Hap concentration evaluated. Therefore, the conditions for the
homogeneous fiber production with a high process yield (higher than 60%) and
minor fiber average diameter were found at 5 wt % of n-Hap and 7000 rpm for
PDLLA, and 5, 10 and 15 wt.% of n-Hap and 6000 rpm for PHB. Through
transmission electron microscopy (TEM) it was observed that n-Hap is mostly
embedded in the fibers, due to the strong interaction with the polymeric
matrices. The thermal stability was not significantly affected for both systems
and the mechanical performance of the PHB systems was improved with the
addition of n-Hap. Additionally, the interaction of the developed fiber mats with
osteoblast-like cells was evaluated to elucidate the influence of polymer matrix
and n-Hap on the cell behavior. In this sense, the PDLLA systems presented
significant higher cell viability than PHB systems after 7 days of incubation.
Moreover, n-Hap promoted a slight increase in the cell metabolic activity.