Abstracts Book - IMRC 2018

• SB2-P037
LOCTITE 3525 AS A LOW-COST, BIOCOMPATIBLE
PHOTOPATTERNABLE CONSTRUCTION MATERIAL FOR
BIOLOGICAL CELLULAR SCAFFOLD
Cindy Viridiana Peto Gutierrez 1
1 Universidad Nacional Autónoma de México, Facultad de Ciencias, Mexico.
In this work, we demonstrate the use of Loctite 3525, a low-cost, off-the-shelf
photosensitive adhesive material with adequate properties for rapidprototyping
of biological scaffolds. Physico-chemical properties important for
this type of application have been assessed and validated: stiffness control by
controlling UV dose, compatibility with spin-coating, direct laser writing for local
polymerization, nanoimprint lithography and soft-lithography techniques. 2D
and 3D molds and microstructures have been successfully fabricated to control
topography of cell substrate. Due to its non adherence to PDMS, it has been easy
to replicate PDMS molds with micron-range features in Loctite 3525, yielding a
micron-range resolution without surface treatment. Furthermore it has proven
an excellent surface material for protein and hydrogels adhesion. Fibroblast
cells response to stiffness change and surface patterning of Loctite 3525 have
been evaluated by immunofluorescence staining of focal adhesion points and
actin cytoskeleton. We believe Loctite 3525 is an excellent alternative for lowcost
cellular scaffold construction.
Acknowledgment:
The authors would like to thank CONACyT for its support (grant 246988) as well
as DGAPA-PAPIIT for the grant IT102017. We also thank José Alfredo Jiménez
Medina for his help with confocal microscopy, Raúl Sánchez-Olvera and Aarón
Cruz-Ramírez for laser ablation of samples and Diego Zamarrón-Hernández for
profilometry.
Keywords: Loctite 3525, rapid photopatterning, biological scaffolds
Presenting authors email: cindypeto@ciencias.unam.mx