Abstracts Book - IMRC 2018

• SB5-P010
EFFECT OF CHITOSAN-COATED GOLD NANOPARTICLES (AUNPS)
ON DENDRITIC CELLS (DC)
Margarita Hernández 1 , Diana Ginette Zarate Trivi�o 1 , Moisés Franco Molina 1 , Cristina
Rodríguez Padilla 1 , Diana Caballero Hernández 1
1
Universidad Autónoma de Nuevo León, Laboratorio de Inmunología y Virología, Mexico.
Nanotechnology employment on devices and medical treatments is a growing
study trend since the past years. Due to its biocompatibility and low toxicity, gold
nanoparticles (AuNps) represent an attractive therapeutic alternative against
cancer, anti-virus, immune-stimulating and for tissue regeneration,
nevertheless, just as conventional drugs, studies for cytotoxicity, side effects,
and immunological response are required. Dendritic cells (DC) represents an
excellent study model to evaluate the immune system reaction to an external
agent; these cells are considered as the bridge between the innate and adaptive
immunity since they can trigger and specific defense through Toll-like receptors
(TLR). The aim of this work is to determinate the effect of AuNps on DC, for this
AuNps were synthesized using the Turkevich method, employing chitosan as
reducing agent. The size was analyzed by dynamic light scattering (DLS) and the
surface plasmon resonance, was determined by UV-vis spectroscopy.
Peripherical blood mononuclear cells (PBMCs) viability was evaluated by flow
cytometry, as well as phagocytic capacity and maturation markers expression
(CD80, CD83, HLA and, CD14). Average size was found in 22nm. The plasmon
surface resonance was observed at 520 nm maximums absorbance. 10, 20, 30
y 40 µM AuNps concentrations were evaluated, and cytotoxicity was not
observed at concentrations below 30 µM, same results were shown in response
to chitosan and chloroauric acid, synthesis precursors.
Keywords: Gold, nanoparticles, dendritic cells
Presenting authors email: m.hernandez.villegas@outlook.com