Abstracts Book - IMRC 2018

• SB6-P066
LIPOSOMAL FORMULATION OF TAXOL: PHYSICAL AND
FUNCTIONAL STABILITY
Jessica Batalla Mayoral 1 , Rocío Guadalupe Casañas Pimentel 2 , Eduardo San Martín Martínez 2
1 Benemerita Universidad Autonoma de Puebla, Complejo Regional Mixteca. Campus Izúcar de
Matamoros, Mexico. 2 Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada,
Legaría, IPN, Laboratorio de Biomateriales, Mexico.
Cancer is a of the main causes of death worldwide. Antitumor drugs are being
nanoencapsulated in polymers and liposomes. One such drug, Taxol, compound
isolated the bark of the Pacific Tejo tree, Taxus brevifolia, shows high
effectiveness in the treatment of cancer of the breast, ovary, lung, skin, head
and neck. However this drug causes hypersensitivity reactions, nephrotoxicity,
and neurotoxicity is incompatible for parenteral administration, also having low
stability and solubility in water which causes the drug to precipitate quickly and
decreases its absorption. There are studies looking to reduce these problems by
the Taxol nanoencapsulation. Liposomes have been used successfully as release
systems of other drugs for the treatment of cancer.
Because of the above, in this research it was developed a system
nanoencapsulation liposome. For nano-encapsulation efficiency of taxol in
liposomes, experiments were performed using high performance liquid
chromatography (HPLC ). A stability study was conducted at different
temperatures (6 °C and -12 °C) and storage time (seven weeks). Characterization
of liposomes was performed in terms of size and charge (Z-potential). The
cytotoxicity of liposomes was evaluated through studies of cell viability by MTT.
Taxol nanoencapsulated in liposomes was quantified by a calibration curve for
Taxol. Nanoencapsulation efficiency of 70% was found, which is consistent with
reports. For cytotoxicity assays were tested liposomes without Taxol (negative
control) and with Taxol at different concentrations. The liposomes, become
unstable after of the storage in the size and consequently affect its cytotoxic
activity. That is, liposomes freshly have a significant cytotoxic effect on the death
of tumor cells (45% to 72h), and when they are stored decreases the cytotoxic
activity due to increased size of the liposomes by aggregation or nucleation at
low temperatures (-12 °C). It is also observed that liposomes change their
surface charge as a function of the storage time thus reduces its cytotoxic,