Abstracts Book - IMRC 2018

• SB6-P032
CYTOTOXICITY ASSAYS ON HELA CELLS WITH GOLD AND SILVER
NANOPARTICLES SYNTHESIZED WITH VITEX MOLLIS FRUIT
HYDROETHANOLIC EXTRACT
Abraham Grijalva Arizmendi 1 , Aaron Alberto Martinez Higuera 1 , Juan Manuel Martínez Soto 2 ,
Cesar Rodríguez Beas 1 , Kareen Krizzan Encinas Soto 3 , Ericka Rodríguez Leon 1 , Ramón Alfonso
Iñiguez Palomares 1
1 Universidad de Sonora, Departamento de Física, Mexico. 2 Universidad de Sonora,
Departamento de Medicina y Ciencias de la Salud, Mexico. 3 Universidad de Sonora,
Departamento de Ingeniería Química y Metalurgia, Mexico.
Synthesis of metallic nanoparticles using plant extract now on days has become
relevant in sustainable chemistry context. The bioactive compounds of extracts
are employed as reducers in the synthesis reaction and stabilize nanoparticles
by adhering to them forming a composite with therapeutic properties. In this
work, we present the synthesis of gold and silver monometallic nanoparticles
(AuNps and AgNps) using a hydroethanolic extract of Vitex mollis fruit as bioreducer.
The nanoparticles are characterized by UV-Vis, IR spectroscopy, singlecrystal
X-ray diffractometer and electron microscopy such as TEM and SEM. The
surface plasmon resonance is located at 429 nm for AgNPs and 540 nm for
AuNPs and the IR spectrum shows the presence of functional groups phytic acid
and fructose even after nanoparticles washing process, indicating those are
stabilizing the material. The size distribution is determined by electron
microscopy (TEM and SEM) obtaining a size mean of 23 nm for AuNPs and 18
nm for AgNPs. X-rays experiments indicate an fcc crystalline structure for both
nanomaterials. They were made viability assay in cervical cancer cells HeLa with
this materials. AuNPs didn’t show cytotoxicity effect at 24hrs, for AgNPs we
determinate the DL50 between 50 and 25 µg/mL.
Acknowledgment: All authors thank The University of Texas at San Antonio
(UTSA) for access to facilities and the use of STEM and Dr. Agustín Gómez and
Ms. Kareen Krizzan Encinas Soto of Departamento de Ingeniería Química y
Metalurgia, Universidad de Sonora for Atomic Absorption Characterization.
The authors would like to thank Dr. Rogerio R. Sotelo-Mundo and Consejo
Nacional de Ciencia y Tecnología (CONACyT) Mexico for grant INFR-2014-01-
225455 to acquire the X-ray single crystal diffractometer Bruker D8
QUEST used in this work.
Keywords: Gold nanoparticles, Silver nanoparticles, Phytic acid
Presenting authors email: xian145@gmail.com