Abstracts Book - IMRC 2018

• SB3-O009
SCAFFOLD FOR TISSUE ENGINEERING FROM EXTRACELLULAR
MATRIX OF MYOCARDIUM
Abraham Israel Muñoz Ruiz 1 , Hector Flores 1 , Amaury Pozos Guillen 1 , Diana M. Escobar García 1
1 Universidad Autónoma de San Luis Potosí, Laboratorio De Ciencias Básicas, Mexico.
Introduction. Heart transplantation remains as the definitive treatment for the
majority of advanced cardiac diseases, however the quantity of donated organs is
limited and the patient require specific conditions for the procedure to be carried
out.Tissue engineering (TE) is a field of medicine that applies principles of the
biological sciences and engineering for the design of tissue substitutes. The main
components of TE are: cells, biomaterials and the combined use of both.
Biomaterials are used to design threedimensional scaffolds that provide a site to
cells for adhesion, proliferation and differentiation. The topography, chemical
composition, microstructure and mechanical properties have a relevant influence
on cellular behavior. In a living organism cells are surrounded by an extracellular
matrix, so it is logical to consider that can act as an ideal scaffold. The objective of
this work is to obtain a scaffold of a decellularized cardiac organ for tissue
engineering.
Methodology. Obtaining Heart. Three male Wistar rats were sacrificed. The rib cage
was subjected to a dissection and the heart was subsequently removed. The heart
was perfused with a pediatric feeding tube with sterile PBS 1X. Decellularization.
The decellularization process was carried out using different solutions (Trypsin, PBS
+ SFB, Triton + ammonium hydroxide, SDS). Extraction of DNA. Cardiac tissue was
mechanically crushed to obtain a representative sample of DNA. A kit of DNA
extraction was used to make a quantification with a NanoDrop equipment. PCR
amplification - GAPDH gene. For amplification was used a comercial kit (Master Mix
+ Primer F + Primer R + Ultrapure water + 250 ng of DNA. The sample was placed
on an agarose gel and read in a photodocumentator.
Results. DNA extraction showed a quantification of 12.4 ng/μl. The PCR
amplification showed that the GAPDH gene was not present.
Conclusion. The low amount of DNA and low expression of GAPDH mean that the
decellularization process was successful. The extracellular matrix of myocardium
could act as a scaffold for tissue engineering.
Keywords: Scaffold, Decellularization, Myocardium
Presenting authors email: a.israel.mr@gmail.com