Abstracts Book - IMRC 2018

• SD7-O020
SYNTHESIS, CHARACTERIZATION AND STUDY OF CYTOTOXICITY
OF Fe3O4 NANOPARTICLES DECORATED WITH Au, Pt AND Pd FOR
MEDICAL APPLICATIONS
Alvaro Angeles 1 , Rodrigo Esparza 2 , Ramiro Pérez 2
1 Centro de Investigación y de Estudios Avanzados del IPN - CINVESTAV, Laboratorio Avanzado
de Nanoscopía Electrónica, Mexico. 2 Centro de Física Aplicada y Tecnología Avanzada UNAM,
Departamento de Nanotecnología, Mexico.
Magnetic nanoparticles (MNPs) have been extensively studied from the
academic point of view until their industrial application; their unique plasmonic
properties allow them to be used for the development of optical applications,
storage devices, sensors, microcircuits and recently in medical applications such
as markers, transport and local drug delivery or drug targeting. In this research
work we present the synthesis by co-precipitation of MNPs Fe 3 O 4 starting from
the salts of FeCl 3·6H 2 O and FeCl 2·4H 2 O, study of the microstructure by scanning
transmission electron microscopy (STEM) in the high-angle annular dark field
(HAADF) and bright field (BF) modes, studies of the magnetic properties and
evaluation of the cytotoxicity of Fe 3 O 4 MNPs decorated with Au, Pt and Pd
respectively in MDKC cell cultures. The obtained results indicate that the
synthesis route by chemical co-precipitation at 80 °C allows obtaining the stable
magnetic phase. The results of XRD-diffraction pattern indicate the reflections
corresponding to the planes (220), (311), (400) and (511) with angles to 30.095,
35.422, 43.052 and 56.942 2θ degrees respectively, related with the cubic phase
type spinel of Fe 3 O 4 . STEM in the high-angle annular dark field (HAADF) and
bright field (BF) modes allowed observing that the Fe 3 O 4 MNPs tend to
agglomerate due to their magnetic nature. In particular, the technique by HAADF
allowed identifying defined hexagonal forms, as well as their interaction with
NPs of Au, Pt and Pd respectively. The magnetic tests carried out on NPs of Fe 3 O 4
and Fe 3 O 4 /Au allowed us to study the effect of stabilization of the magnetic
phase in contact with Au NPs. Finally, the studies of cytotoxicity allowed knowing
the effect of cell death by necrosis and apoptosis that promote the NPs of Fe 3 O 4
and decorated with Au, Pt and Pd in the MDCK type cells.
Acknowledgment:
The authors thank to Adolfo Tavira Fuentes for the support in X-ray diffraction
(XRD) Department of Electrical Engineering (SEES). Also, the authors would like