Abstracts Book - IMRC 2018

• SB6-P096
GOLD AND AU@AG NANOPARTICLES SYNTHESIZED WITH RUMEX
HYMENOSEPALUS EXTRACT: A CYTOTOXIC STUDY IN HELA CELLS
Mauro Villalobos Noriega 1 , Aaron Alberto Martinez Higuera 1 , Juan Manuel Martínez Soto 2 ,
Ericka Rodríguez Leon 1 , Ruben Mendoza Cruz 3 , Yedith Soberanes 4 , Roberto Carlos Carrillo
Torres 1 , José Manuel Galvan Moroyoqui 2 , Ramon Iñiguez Palomares 1
1 Universidad de Sonora, Departamento de Física, Mexico. 2 Universidad de Sonora,
Departamento de Medicina y Ciencias de la Salud, Mexico. 3 The University of Texas at San
Antonio, Department of Physics and Astronomy, United States. 4 Universidad de Sonora,
Departamento de Polímeros y Materiales, Mexico.
Rumex hymenosepalus is an endemic plant northern Mexico and southern
U.S.A. rich in antioxidant compounds including catechins and stilbenes. The
synthesis process of gold and core@shell nanoparticles is described based on
the use of Rumex hymenosepalus root extract as a bioreductor agent. The
cytotoxicity of these materials was evaluated in human cervical cancer cells
(HeLa). The products obtained were characterized by Transmission Electron
Microscopy (TEM), Scanning TEM (STEM) with high-angle annular dark-field
imaging (HAADF), UV-VIS Spectroscopy, Infrared Spectroscopy (IR) and Dynamic
Light Scattering (DLS). TEM results indicate that nanoparticles are mainly quasispherical
with sizes between 35-40 nm. The existence of core@shell structure
was confirmed by STEM. IR of the nanomaterials indicates that the system is
stabilized by the polyphenolic molecules present in the extract. Crystalline
structure of nanomaterials determined by HRTEM and X-ray diffraction
correspond to face centered cubic structure (fcc). Cytotoxicity evaluation in HeLa
cells of these materials was carried out by counting with Trypan Blue. 24
hours results showed a LD50 between 50-100 μg/mL for the Rumex
hymenosepalus extract, while the gold and Au@Ag nanoparticles showed no
significant toxicity. However, a confocal microscopy study revealed the presence
of modifications to the supporting structures of the cells due to the gold
nanoparticles
Acknowledgment:
All authors thank The University of Texas at San Antonio (UTSA) for access to
facilities and the use of STEM and Scanning Electronic Microscopy Laboratory of
the University of Sonora. The authors would like to thank Dr. Rogerio R. Sotelo-
Mundo and Consejo Nacional de Ciencia y Tecnología (CONACyT) Mexico for