Abstracts Book - IMRC 2018

• SB6-P005
BACTERIAL INACTIVATION BY THE EFFECT OF NANOCOMPOSITE
BASED ON ZINC OXIDE NANOPARTICLES
Jesica Yanina Pereyra 1 , Diego F. Acevedo 1 , Edith Ines Yslas 1 , Cesar Alfredo Barbero 1
1 Instituto de Investigaciones en Tecnologias Energeticas y Materiales Avanzados (UNRC-
CONICET), Chemistry, Argentina.
In this work, the synthesis, characterization and application as bactericidal
material of the nanocomposite is shown. The hydrogel was synthesized by
copolymerization between N-isopropylacrylamide (NIPAM) and
hydroxiethylmetha-acrylate (HEMA) (PNIPAM-co-10% HEMA). Zinc oxide
nanoparticles (NPsZnO) were synthesized insitu inside of the biocompatible
hydrogel the precursor salt zinc nitrate. The zinc nitrate was allowed to diffuse
inside the hydrogel for 24 hours. Then the swelling hydrogel was immersed in
KOH solution and then the nanoparticles were obtained by using microwave.
The antimicrobial activity of the nanocomposite was evaluated using a Gram
negative (-) bacterium as the Pseudomonas aeruginosa model. This action was
studied by means of the diffusion method in agar, which was evidenced by the
formation of inhibition halos. The size of the inhibition halos increases whit the
incubation time (24-48 h) suggesting an important antibacterial effect of the
nanocomposite. From the results of viability and inhibition halos using as a
control group the hydrogel and as a group treated the nanocomposite at
different incubation times a marked antibacterial action of the Zinc oxide
nanocomposite (PNIPAM-co-10% HEMA) dependent on time was observed. In
conclusion, these results reveal that zinc oxide generated inside of PNIPAM-co-
10%HEMA can be used as intelligent hydrogel to kill bacteria.
Acknowledgment:
D.F. Acevedo, E. I Yslas and C.A. Barbero, are permanent research fellows of
CONICET. J Pereyra thanks CONICET for a graduate fellowship. The funding of
FONCYT (PICT 2013-2716-PICT 2016 0417), CONICET (PIP 2014-2016, No.
11220130100663CO), SECYT-UNRC are gratefully acknowledged.
Keywords: Bactericide, Hydrogel, Zinc Oxide
Presenting authors email: cesarbarbero@gmail.com