Abstracts Book - IMRC 2018

• SB2-P018
TOXICOLOGICAL EVALUATION OF SPIONS ON BRAIN
MICROVASCULAR ENDOTHELIAL CELLS
Lorena Garate Velez 1 , Claudia Escudero Lourdes 2 , José Luis Rodríguez López 1
1 Instituto Potosino de Investigación Científica y Tecnológica A.C., Materiales Avanzados,
Mexico. 2 Universidad Autónoma de San Luis Potosí, Ciencias Químicas, Mexico.
Anthropogenic iron nanoparticles (NPs), such as super-paramagnetic iron
nanoparticles (SPIONS, mainly in magnetite phase, Fe3O4) are released to the
environment as a result of the abrasion of combustion cylinders and vehicle disc
brakes, therefore they can be found in dust samples high density traffic area.
Fe3O4 NPs, have been found in human brain tissue, but unlike biogenic
magnetite, these NPs have a mean diameter of 18 nm and seems to get directly
into the brain through the olfactory nerve or it crossing the blood-brain barrier
(BBB). Their presence in this tissue has been associated with neurodegenerative
diseases such as Alzheimer due they are inducers of oxidative stress. A wide
spectrum of studies have evaluated the toxicity of Fe3O4 NPs that are
superficially modified to be used for drugs delivery purposes, however there is
scarce information about the toxicity of naked magnetite NPs on cells that form
the BBB, that could contribute to explain their presence in brain tissue and
evaluate their possible role on neurodegeneration.
In this work we synthesized, characterized and evaluated the Fe3O4 NPs toxicity
on an in vitro monolayered BBB model using primary rat brain microvascular
endothelial cells (rBMVEC). Fe3O4@SiO2 NPs system was used for comparison
purposes against naked SPIONS. In the characterization of structural and
superficial composition, the addition of coating in Fe3O4@SiO2 had small
variations in results. Both samples presented semi-spherical morphology with
mean diameter around 11.4 nm and 17.5 nm for Fe3O4 and Fe3O4@SiO2 by SEM,
respectively.
The cellular toxicity, oxidative stress and internalization were evaluated on
rBMVECs through LDH release, MTS reduction, 2’-7’ dichlorofluorescin (H2DCF)
oxidation and TEM, respectively. Results showed that both kind of NPs, naked
and covered with SiO2 get internalized into the cells, however naked Fe3O4 NPs
induced stronger toxic effects on rBMVECs in terms of viability and oxidative
response.