Abstracts Book - IMRC 2018

• SB6-P038
In vitro BIOCOMPATIBILITY OF GELATIN-BASED HYDROGEL FILMS
IN MUCUS PRODUCING HUMAN COLONIC CELLS
Jorge Armando Jiménez Avalos 1 , Rogelio Rodríguez Rodríguez 1 , Marisela González Avila 1 , Hugo
Espinosa Andrews 2 , Zaira Y. García Carvajal 1
1 Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Medical and
Pharmaceutical Biotechnology Unit, Mexico. 2 Centro de Investigación y Asistencia en
Tecnología y Diseño del Estado de Jalisco, Food Technology Unit, Mexico.
Current models for study intestinal functions are limited, or lack of physiological
relevance to in vivo conditions when using conventional cultures (2D) or animals.
An interesting option to study these functions relies on the use of threedimensional
(3D) cell cultures, who has raised as an interesting system to bridge
the gap between conventional monolayer cultures and in vivo models, providing
a cellular environment more consistent with that in vivo.
To achieve this, the use of polymers, synthetic or naturals, are thought as
promising candidates to develop scaffolds for intestinal tissue engineering
applications.
In this study, we investigated the porosity and interconnectivity properties, and
the in vitro biocompatibility of a gelatin-based hydrogel in a human colon
carcinoma cell line (HT29-MTX) model.
We synthetized the gelatin-based hydrogel films by a solvent cast process, in
order to generate thin hydrogel films. Porosity and 3D structure were
determined by Scanning Electronic Microscopy (SEM) and by Confocal Laser
Scanning Microscopy (CLSM) employing rhodamine B staining method,
respectively. Qualitative evidence indicated an average pore diameter of 5 µm.
The hydrogel films showed biocompatibility using LIVE/DEAD
cytotoxicity/viability assay in HT29-MTX cell line cultured onto hydrogel films at
day 1, 3, 5, 7 and 14. To demonstrate the cell adhesion capability to hydrogel
films, cells were seeded and immunostained in order to identify molecules
involved in cell-extracellular matrix adhesion, such as vinculin.
Our preliminary results our in vitro studies suggest that gelatin-based hydrogel
films may have further applications as a 3D scaffold for human intestinal cells.