Abstracts Book - IMRC 2018

• SB6-O006
DEVELOPMENT AND IN VITRO EVALUATION OF
POLYCAPROLACTONE-GELATIN ELECTROSPUN MATRICES AS
POTENTIAL SCAFFOLDS FOR WOUND TREATMENT
Gina Prado Prone 1,2 , Maasoomeh Bazzar 3 , Phaedra Suriel Silva Bermudez 2 , Maria Letizia
Focarete 3 , Jorge A. García Macedo 4 , Adriana Hernandez Rangel 2,5 , Angelica Fernandez Puron 2,6 ,
Sandra Elizabeth Rodil 7 , Cristina Velasquillo 8
1 Instituto de Investigaciones en Materiales, Posgrado en Ciencia e Ingeniera de Materiales,
Mexico. 2 Instituto Nacional de Rehabilitacion, Unidad de Ingeniería de Tejidos, Terapia Celular
y Medicina Regenerativa, Mexico. 3 Alma Mater Studiorum - Università di Bologna, Dipartimento
di Chimica Giacomo Ciamician, Italy. 4 Instituto de Física - Universidad Nacional Autónoma de
México, Departamento de Estado Solido, Mexico. 5 Centro de Investigación y de Estudios
Avanzados del IPN - CINVESTAV, CINVESTAV-Queretaro, Mexico. 6 Facultad de Química, UNAM,
Facultad de Química, Mexico. 7 Instituto de Investigaciones en Materiales, Departamento de
Baja Dimensionalidad, Mexico. 8 Instituto Nacional de Rehabilitacion, Subdirección de
Investigación Tecnológica, Mexico.
Major burns causing wide and deep wounds require specialized treatment to
heal. Autologous skin transplantation is the “gold standard” in these cases;
however, in the case of extensive burns there is little healthy tissue remaining
for autologous skin grafts. One avenue to overcome this shortage is the
development of scaffolds able to simultaneously work as protective covers and
carriers to transport cells into wound beds; these scaffolds must be
biocompatible and appropriate for cell culture. Polycaprolactone (PCL) is a
synthetic polymer widely used in tissue engineering due to its biocompatibility,
low melting point, slow biodegradation rate in comparison to natural polymers
and appropriate mechanical properties; however, PCL is hydrophobic and
biologically inert. Combining PCL with a biofunctional polymer such as gelatin
(GEL) presents the opportunity to overcome PCL hydrophobicity and lack of
biofunctionality. Gelatin is a natural polymer obtained by partial hydrolysis of
collagen, the most abundant protein in the dermis extracellular matrix (ECM).
One way of combining these two polymers into a biofunctional scaffold with
appropriate mechanical properties is by electrospinning technique, which offers
the advantage of obtaining matrices with fibrillar structures that resemble the
ECM morphology. In the present study, we developed PCL-Gel electrospun
matrices (mats) with different GEL concentrations (5, 15, 30 and 45 wt.%) using
acetic acid as a green sole solvent for the electrospinning solutions. Mats
morphology, elemental composition, chemical structure, water wettability and
gelatin release in aqueous media were characterized by Scanning Electron