Abstracts Book - IMRC 2018

• SB6-O025
NOVEL POLYMERIC MATERIALS FOR SOLID- PHASE EXTRACTION
OF SARCOSINE
Soane Fernández Puig 1 , Miguel Alberto López Sánchez 1 , Jheny Adriana Rodríguez Mendez 2 ,
Arístides Camilo Valdés González 3 , Abraham Ulises Chávez Ramírez 1
1 Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Electrochemistry, Mexico.
2 Universidad Autónoma de Querétaro, Faculty of Medicine, Mexico. 3 Universidad de La
Habana, LUCES, Cuba.
Prostate cancer (PCa) is an important cause of mortality and morbidity of men.
The prostate specific antigen (PSA) normally used as a marker can only be
detected in blood with low sensitivity an specificity. Recently, several metabolites
presented in body fluids such as serum or urine have been investigated as new
putative biomarkers in relation to PCa [1]. Sarcosine [2]has received attention
as a potential PCa biomarker, since it has found that its concentration levels
increased with cancer progression. In this work, highly selective molecularly
imprinted polymers (MIPs) for sarcosine pre-concentration were developed and
its use as solid-phase extraction (SPE) sorbent material was demonstrated.
These MIPs were prepared was prepared by a very simple procedure using
metacrilic acid as funtional monomers, acetonitrile as porogen and ethylene
glicol dimethacrylate as cross-linking agent. The synthetized materials were
charaterized by SEM and irregular morphology was observed in both materials,
particle size was in the range of 38 to 250 microns.
The polymer was tested in batch experiment in order to evaluate its binding
properties: the maximum extraction pH, the sorption capacity, as well as the
sorption capacity of the MIP with respect to the non-printed one were
determined. The developed polymer was able to detect low sarcosine
concentrations in the range of 10 -1 to 1 ng/L in synthetic samples and these
results support a potential material to be tested in biological samples for PCa
diagnostics.
[1] A.P. Khan, T.M. Rajendiran, A. Bushra, I.A. Asangani, J.N. Athanikar, A.K.
Yocum, R. Mehra, J. Siddiqui, G. Palapattu, J.T. Wei, G. Michailidis, A. Sreekumar,
A.M. Chinnaiyan, The Role of Sarcosine Metabolism in Prostate Cancer
Progression, Neoplasia 15(5) (2013) 491-IN13.
[2] A. Sreekumar, L.M. Poisson, T.M. Rajendiran, A.P. Khan, Q. Cao, J. Yu, B.
Laxman, R. Mehra, R.J. Lonigro, Y. Li, Metabolomic profiles delineate potential