Abstracts Book - IMRC 2018

• SB3-O001 Invited Talk
POROSITY AND THERMAL STABILITY OF MOLECULARLY
IMPRINTED GELATIN-BASED COMPOSITE HYDROGEL FOR TISSUE
ENGINEERING APPLICATIONS.
Rogelio Rodríguez Rodríguez 1 , Hugo Espinosa Andrews 2 , Cristina Velasquillo Martínez 3 , Yaaziel
Melgarejo Ramírez 3 , Jorge Armando Jiménez Avalos 1 , Inés Jiménez Palomar 4 , Zaira Yunuen
Garcia Carvajal 1
1 Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Medical and
Pharmaceutical Biotechnology, Mexico. 2 Centro de Investigación y Asistencia en Tecnología y
Diseño del Estado de Jalisco, Food Technology, Mexico. 3 Instituto Nacional de Rehabilitacion,
Tissue Engineering, Cell Therapy and Regenerative Medicine, Mexico. 4 Additive Manufacturing
Laboratory, , Mexico.
Porosity is an essential property in the fabrication of scaffold for tissue
engineering applications. Pores should be large and interconnected to allow cell
growth and cell migration, facilitating the transfer and permeation of solutes as
nutrients, metabolites, oxygen, waste, drugs, among others. Water uptake
increases the size pore and reduces the mechanical properties of hydrogels,
compromising the structural stability of the scaffold. The thermogravimetric
analysis allows knowing the temperature at which a material begins to
decompose as well as the magnitude of mass loss. High thermal stability
indicates high polymer resistance to a thermal process such as steam
sterilization process. The most common techniques used to create porosity
including cryogenic and freeze-drying process, depending on the material used
to fabricate the scaffold. The aim of the present investigation was fabricated
gelatin-based polymer scaffolds using a cryogenic process following a freezedrying
process and chemical treatment based on molecular imprinting
technology (MIT). In order to evaluate the relationship between the impacts of
scaffold preparation MIT technique in the pore size and thermal stability of the
hydrogels, we analyzed morphology using confocal microscopy, water uptake,
thermogravimetric analysis and rheological properties. Composite hydrogels
showed a highly hydrophilic behavior, with a water uptake capacity 9 to 13 times
their mass. Morphology of composite hydrogels showed high porosity using
confocal microscopy (> 100 um). The thermogravimetric analysis displays
different decompositions stages, with an onset decomposition temperature
below to 206 °C Along the entire frequency range, G’ modulus higher than G’’
modulus, indicating a predominantly solid viscoelastic. At lower frequency (0.1
rad/s), G’ modulus and G´´ modulus showed values of 5588 and 419 Pa,