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Orange Peel Waste as a Potential Substrate for Pectinase Production by
Aspergillus Niger in Solid State Fermentation
Lim Loo Yee
Supervisor: Dr. Nazaitulshila binti Rasit
Bachelor of Technology (Environment)
School of Ocean Engineering
Universiti Malaysia Terengganu
Pectinase enzyme is the eco-enzyme produced from the fermentation of vegetables
or fruit wastes with Aspergillus niger. Application of pectinase enzyme on pretreatment
of textile wastewater is efficient, eco-friendly and low-cost. The
characterization of pectinase enzyme was conducted based on pH, Chemical Oxygen
Demand (COD) and pectinase activities. Besides, optimization of pectinase enzyme in
SSF was conducted based on incubation time, temperature, substrate concentration,
inoculum size and pH. The parameters were tested from the pre-treatment of textile
wastewater with pectinase enzyme which included total dissolved solids (TDS),
Biochemical Oxygen Demand (BOD5) and COD. The highest pectinase activity showed
extracted enzymes were optimally energetic in pH of 5.5, 35°C, 96 hours, 10 g
substrate concentration and 6ml inoculum size. 10% dosage of dilution pectinase
enzyme was used to test the efficiency on pre-treatment of textile wastewater. This
study was showed that high potential application of pectinase enzyme on the
treatment of textile wastewater.
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