13.05.2018 Views

merged

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Production and Purification of Polysaccharide from Bacteria,<br />

Brevundimonas sp. Associated with Horseshoe Crab, Tachypleus Gigas<br />

Syafawani Binti Mokhtar<br />

Supervisor: Assoc. Prof Dr. Ahmad Shamsuddin Bin Ahmad<br />

Bachelor of Science (Marine Biology)<br />

School of Marine and Environmental Sciences<br />

Polysaccharides are secondary metabolites that consist of many monomers link by<br />

glycosidic bond. It has been widely used in pharmaceutical and medical purposes.<br />

Therefore, the aim of this study is to investigate the production and purification of<br />

polysaccharides from bacteria, Brevundimonas sp. associated with horseshoe crab,<br />

Tachypleus gigas. One species of bacteria was selected from Makmal Belangkas, UMT.<br />

Biochemical, antibacterial, morphological and physiological test were conducted. The<br />

bacteria were identified by vitek2 machine. For production of polysaccharide, the<br />

culturing, dialysis, ethanol precipitation and hydrolysis process were done. Sugar<br />

composition was identified by paper chromatography (PC) and High-Performance<br />

Liquid Chromatography (HPLC) with 1.2 ml/min flow rate. Results show, the bacteria<br />

is gram-negative bacteria, Brevundimonas sp. and able to produce 79.5 mg/L of<br />

polysaccharides. Results from both chromatography techniques show two types of<br />

sugar composition, identified as rhamnose and glucose indicating the potential of the<br />

bacteria, Brevundimonas sp. to produce some sugar.<br />

1838 | UMT UNDERGRADUATE RESEARCH DAY 2018

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!