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Morphological Characteristics and Genetic Variability of Stingless<br />

bees (Geniotrigona thoracica) in Terengganu<br />

Vinailosni A/P Amirthalingam<br />

Supervisor: Dr. Wan Bayani Bt. Wan Omar<br />

Co-Supervisor: Assoc. Prof. Dr. Wahizatul Afzan Bt. Azmi<br />

Bachelor of Applied Science<br />

(Biodiversity Conservation and Management)<br />

School of Marine and Environmental Sciences<br />

In determination of the species, the morphology part plays a vital role along<br />

with molecular studies. Besides, genetic variability is very crucial, as it<br />

determines the ability of the species, to adapt to the changes of the<br />

environment. The aims of this study were to determine the morphological<br />

characteristics of domesticated G. thoracica among two different sites, Sekayu<br />

Recreation Park and Tembila, Besut in Terengganu and to determine the<br />

genetic variability of the domesticated G. thoracica using RAPD as the molecular<br />

marker. By using Dino-lite, with stereo-microscope, the morphormetric<br />

measurements of the stingless bee were taken and recorded. Then, the data<br />

analysis was conducted using multivariate analysis. From the data analysis, it<br />

was seen that there were significant differences between the two sites. The<br />

DNA of G. thoracica were extracted using DNeasy tissue kit (Qiagen TM ) and the<br />

PCR was run using RAPD primer, which were OPA 6, OPA 11 and OPA 17.<br />

1776 | UMT UNDERGRADUATE RESEARCH DAY 2018

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