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Bacteria Isolation From Skin, Gills And Water When Treated With Different<br />

Diet Studied In Oreochromis sp.<br />

Nur Syuhada Binti Rosli<br />

Supervisor: Dr. Nor Omaima Harun<br />

Bachelor of Science (Biological Sciences)<br />

School of Fundamental Science<br />

The aim of this study is to determine the microbial community and the immunity of<br />

fish when treated with different diets. 75 fish were treated with different diets in five<br />

tanks. The treatment are by having the commercial pellet to be added whether with<br />

oligochaete (Eisenia foetida), oyster mushroom (Pleurotus sajor-caju), pitcher plant<br />

(Nepenthes gracilis) or combination of all of the ingredients. The fish is collected<br />

randomly to analyze the bacteria in each week. The results of identification tests<br />

showed 190 bacteria colonies from 12 species have been identified by using traditional<br />

biochemical test. Three species are rarely found on fish. The bacteria are vary,<br />

encompasses Gram-positive bacteria and Gram-negative bacteria with different<br />

morphology and characteristics. In this study, fish have observed to secrete more<br />

mucus (protein) than bacteria colony isolated to avoid invasion of pathogens. Thus,<br />

the different diet utilization in fish have role in changing the microbial community and<br />

increase the immunity of fish.<br />

388 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018

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