merged

Enzymatic Activities in Mangrove Fungus Tricoderma Atroviride as
Response to Chromium Toxicity
Nur Ain Shahirah binti Noor Hisham
Supervisor: Assoc. Prof. Dr. Mariam bt Taib
Bachelor of Science (Biological Sciences)
School of Fundamental Sciences
Contamination of Chromium, Cr in the environment is one of the major areas in
concern. Cr used in industrial component function including wood tanning. Cr (III)
which is in trivalent is vital to human and animal while Cr (VI) is toxic and could cause
cancer. Trichoderma atroviride is one of the bioremediation agents and when
T.atroviride exposed to heavy metal, reactive oxygen species (ROS) activated to
detoxify heavy metal become less toxic material. Catalase (CAT), Guaiacol peroxidase
(GPX) and ascorbate peroxidase (APX) are antioxidative enzymes that play roles in
scavenging free radicals. According to the result, there were positive respond of
T.atroviride growth in Cr. The dry weight of mycelia dropped for each concentration.
While HRPs content rose at beginning and dropped at 400 μM. For CAT and GPX
activities indicated the highest at 100 μM and APX shown at 300 μM.
322 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018