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EVALUATION OF OXIDATIVE STRESS IN Meretrix meretrix USING<br />

NITRIC OXIDE SYNTHASE AS BIOMAKER<br />

LAW JIA LEE<br />

SUPERVISOR: DR. SITI NURTAHIRAH BINTI JAAFAR<br />

CO-SUPERVISOR: DR. SUVIK ASSAW<br />

BACHELOR OF SCIENCE (MARINE BIOLOGY)<br />

SCHOOL OF MARINE AND ENVIRONMENTAL SCIENCES<br />

Nitric oxide (NO) is one of the reactive oxygen species (ROS) that induce<br />

oxidative stress in organisms during metabolism process and immunological<br />

reaction. NO production is regulated by nitric oxide synthase (NOS) in<br />

mechanism action of oxidative stress. This study aimed to evaluate the<br />

oxidative stress by using NOS as biomarker in hard clams, M. mertrix collected<br />

from Setiu Wetlands. NOS activities were determined and measured by using<br />

colorimetric method spectrophotometrically. Oxidation of protein thiols was<br />

studied by using one-dimension gel electrophoresis (SDS-PAGE). The results<br />

showed significant interaction between the level of NO activities from Station 2<br />

(p

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