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Effect of Dietary Supplement on Organs Study in Red Tilapia<br />

(Oreochromis sp.)<br />

Asma Hanif Binti Mohd Hanafi<br />

Supervisor: Dr. Nor Omaima Binti Harun<br />

Bachelor in Science (Biological Sciences)<br />

School Of Fundamental Science<br />

Rapid growth of aquaculture industry had increased the demand of aquaculture<br />

production where availability of protein sources like fishmeal is scarce and expensive.<br />

This study aim to determine the positive effect of dietary supplements; Pleurotus<br />

ostreatus (oyster mushroom), Eisenia foetida (earthworm), Nepenthes gracilis (pitcher<br />

plant),a combination of three types diet and commercial pellets as a control to organs<br />

of red tilapia through histological changes of gills, liver, and small intestine by using<br />

H&E hemoxylin for 28 days. Different changes in different organs was appeared after<br />

28 days. The liver alteration showed vacuolation, necrosis, hemorrhage, and presence<br />

of glycogen in the hepatocytes. The small intestine showed necrosis of mucosa,<br />

formation of lipid, folded and loose of the lamina propria in different diets. In<br />

conclusion, earthworm diet was the best choice alternative diet supplement in<br />

improving immunity against pathogen and enhancing nutrient of fish.<br />

Keywords: Fish, histology, Eisenia foetida, Pluerotus ostreatus, Nepenthes gracilis.<br />

gills, liver small intestine.<br />

386 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018

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