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Investigation of Free 3-Mcpd Compound Cytotoxicity Mechanism in
Human Colon Cell (Hct 116) Via Mapk Pathway
Nurnadhirah Zulaikha binti Mohd Nizam
Supervisor: Dr Nurul Huda binti Abdul Kadir
Bachelor of Science (Biological Sciences)
School Of Fundamental Science
Colon cancer was reported as second common cancer among males and the third
common cancer among females, in Malaysia. Food contaminants such as 3-
monochloropropane-1, 2 diol (3-MCPD) are reported to be detected in foods
manufactured from oils and fats. Cytotoxic assay were evaluated by using
AlamarBlue assay. High cytotoxicity effect on HCT 116 cell line was shown when
the cell’s viability was significantly decreased at highest concentration (100mM).
To measure ROS activity, a fluoregenic dye, DCFDA was used. Western blot result
has shown the ERK1/2, p-ERK, BCL-2 protein expression after treated HCT-116
with 3-MCPD were upregulated after 48 hours, while for caspase-3 it was
upregulated and down regulated after 48 hours. To summarize, this study showed
that the high concentration of free 3-MCPD compound have a high toxicity towards
HCT 116. Thus, it is concluded that this compound could be potentially be as a
cancer causing agent.
390 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018