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EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY ON
CRUDE FRACTIONS FROM TWIGS OF AVICENNIA OFFICINALIS
KHAW TYNG TYNG
Supervisor: DR. SUVIK ASSAW
BACHELOR OF SCIENCE (MARINE BIOLOGY)
SCHOOL OF MARINE AND ENVIRONMENTAL SCIENCE
Previous studies showed that mangrove plants are endowed with various
bioactive compounds with potent antibacterial and antioxidant properties. This
study was developed to determine the antibacterial and antioxidant properties
on the crude fractions from Avicennia officinalis twigs. The selected fractions
were evaluated for antibacterial activity using well-diffusion assay against three
Gram-negative and positive bacteria. Meanwhile, antioxidant activity of the
obtained fractions was evaluated using DPPH radical scavenging assay. The six
fractions exhibited selective antibacterial activity against Gram-positive bacteria
and no inhibition against Gram-negative bacteria. Fraction 7 and 8 showed
strong antibacterial activity with minimum inhibition concentration (MIC) values
of 0.156-2.50 mg/mL. Potent antioxidant activity showed by fraction 9 with
50% of inhibition concentration (IC50) at 0.7 mg/mL. The active fractions will
be further isolated to target the pure metabolites that might responsible to the
activity. The results obtained will enhance the knowledge on potential uses of
our local plant sources.
1791 | UMT UNDERGRADUATE RESEARCH DAY 2018