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Antioxidant Enzymatic Activity in Mangrove Fungus Trichoderma
Atroviride as Response to Iron Toxicity
Farha Yasmin binti Faris Taufeq
Supervisor: Assoc. Prof Dr. Mariam bt Taib
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Heavy metals are becoming a global issue due to its harmful effects to the biodiversity.
They also caused plants to stress up and produce reactive oxygen species (ROS). In
this study, mangroves fungus T. atroviride was treated with iron (Fe) in order to study
its antioxidant enzymatic activity. Previous study showed that T. atroviride is known
to be one of the best biological agents as they are able to detoxify heavy metals. The
objective of this study was to determine the enzymatic activities of Catalase (CAT),
Guaicol Peroxidase (GPX) and Ascorbate peroxidase (APX) in fungus T. atroviride upon
exposure to different concentrations of iron (Fe). The results showed that the amount
of peptides expressed by T. atroviride is increasing. In enzymatic assays, the activity
of APX is increasing along with the increasing Fe. However, the highest activity in CAT
is 400 μM while GPX is 100 μM and it decreasing.
321 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018