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Cytotoxicity Effect of Human Colon Cell Line (Hct 116) After Induced With
Glycidamide via Mitogen-Activated Protein Kinase (Mapk) Pathway
Nurshahirah Ashikin binti Moidu
Supervisor: Dr. Nurul Huda binti Abdul Kadir @ Abd Rahman
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Glycidamide is metabolite of Acrylamide, type of food carcinogen found in heating
process of carbohydrate-rich food at high temperature such as French fries. An
experiment to assess the cytotoxicity effect of Human Colon Cell Line (HCT 116) after
induced with glycidamide was conducted. Result showed cells viability reduced after
treated with different concentration of glycidamide and was evaluated using
AlamarBlue assay. Glycidamide showed time and dose-dependent inhibition on the
growth of cells with IC50 values of 1.55mM, 1.00mM and 1.50mM. This is due to
production of ROS that lead to oxidative stress and apoptosis. Amount of ROS were
measured by fluorometric assay using DCFDA. In order to confirm the apoptotic event,
protein expression of ERK 1/2, p-ERK, BCL2, and Caspase-3 using western blotting
were done. Results have shown that up-regulation of BCL2 and Caspase-3 at 24 and
48 hours might induce apoptosis to cells. Thus, glycidamide could potentially induce
cancer.
393 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018