merged

Indentification of Microalgae Isolated From Morib
(Polychaetes-Rich Habitats)
Wan Nur Syarafina Binti Wan Ahmad
Supervisor: Dr. Malinna binti Jusoh
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Polychaetes are one of the most vital groups of marine benthic animals which are
deposit feeders, swallow mud and feed on the microalgae that attached to the
particles. In order for the polychaetes to feed on microalgae, the habitats of
polychaetes are likely to have variety type of microalgae that live together. The
objective of this study was to isolate and identify the microalgae from the habitat of
polychaetes by using 18s rDNA. Mud sample from the polychaetes- rich habitat were
taken from Morib and cultured on F/2 liquid media before transferred to F/2 agar
media. DNA was extracted from the single colony culture and PCR was done using
EuKA and EuKB primers. Expected band of 2000bp was excised and sent for
sequencing. The sequence BLAST analysis showed highest similarity as Stichococcus
bacillaris sp. This result was supported by morphology observation under the
microscope. Thus, there was a relationship between polychaetes and microalgae.
352 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018