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Cytotoxic Effect of Namalycastis sp. Crude Extract on 3t3 Fibroblast
Cell Growth
Muhammad Faiq Najmi Bin Tajudin
Supervisor: Dr. Maziah binti Mohd Ghazaly
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Namalycastis sp., a family of polychaete which is known for its repair and cell
regeneration ability, potential for medicinal uses is unknown and there is no data on
its effects towards normal cells. Current study was conducted to investigate the
cytotoxic effect of Namalycastis sp. crude extract (NCE) treated on 3T3 fibroblast cell
for 24 hours. Different concentrations (0-8000 µg/mL) of NCE were used. The results
were as follow; MTT assay proves that NCE enhanced 3T3 fibroblast cell proliferation.
Moreover, IncuCyte analysis shows increase percentage of cell viability on treated
cell as compared to control. Finally, migration assay shows that treated cells
completely proliferate in less than 20 hours. Thus, NCE is non-toxic towards cell and
able to enhanced growth and proliferation of cells. These findings can be used to
determine the medicinal potential of Namalycastis sp. on the severely debilitating
diseases such as Parkinson’s, Alzheimer’s and dementia.
Keyword: Namalycastis sp., cytotoxicity, cell growth, proliferation, 3T3 fibroblast cell
374 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018