merged

Effect of cooling rates and immersion temperature on cryopreserved
sperm motility of Malaysian mahseer (Tor tambroides)
Rahimah Binti Razali
Supervisor: Dr Ivan Koh Chong Chu
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Malaysian Mahseer (Tor tambroides) is a highly valued species with decreasing wild
populations due to over-exploitation. Sperm cryopreservation will help to establish the
seed production of Malaysian Mahseer. The aim of this study is to investigate the
effect of cooling rates and immersion temperature on the sperm cryopreservation of
the Malaysian Mahseer. Cooling rates were investigated by cooling straws at 5
different heights from liquid nitrogen (LN) surface (2.5cm, 5.0cm, 7.5cm, and 12.5cm)
while straws were immersed in LN at 5 different temperatures (-30°C to -70°C) to
determine optimun immersion temperature. The results show that optimum cooling
rates for Malaysia Mahseer ranged from 4.05-9.60°C min -1 which corresponds to
heights of 10-12.5cm. The optimum immersion temperature was -60°C with highest
post-thaw motility at 31.33±16.17%. In conclusion, we had optimized the cooling
rates and immersion temperature of Malaysian Mahseer.
530 | UMT UNDERGRADUATE RESEARCH DAY 2018