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Genetic variation of Malabar Trevally, Carangoides malabaricus in<br />

Malaysia using mitochondrial DNA cytochrome c oxidase subunit I (COI)<br />

Tee Say May<br />

Supervisor: Dr Tun Nurul Aimi Binti Mat Jaafar<br />

Bachelor of Science in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

Carangoides malabaricus is one of the most commercialized trevally species in<br />

Malaysia. Nevertheless, their genetic status is still remained unknown along with<br />

constant fear of over-exploitation. Consequently, this study aims to identify their<br />

genetic variation and population structure in Malaysia using cytochrome c oxidase<br />

subunit I (COI) gene. The 652bp of COI gene from 67 specimens were amplified and<br />

the finding reveals 39 haplotypes with haplotype 2 e<strong>merged</strong> as the most shared<br />

haplotype within 22 specimens. The average haplotype diversity (h) and nucleotide<br />

diversity (π) were 0.89000 and 0.01091 respectively with Sibu showed the lowest<br />

among all localities. The Neighbour Joining (NJ) and Maximum Likelihood (ML) trees<br />

showed 2 clusters with COI nucleotide divergence of 1.4%. Additionally, the FST=<br />

0.10366, FCT= 0.12197 and FSC= -0.02086 values were proven to be low which indicate<br />

low genetic divergence of C. malabaricus in Malaysia due to high gene flow of these<br />

migratory species.<br />

567 | UMT UNDERGRADUATE RESEARCH DAY 2018

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